FastQCFastQC Report
Fri 10 May 2024
EGAF00004945429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004945429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296802068
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGCTC8159906827.492755879315506No Hit
GTCCTTCT7957901526.812149772487437No Hit
AAGACGGA7862425226.490466366966146No Hit
TCTTAAAG5177188917.443237289033984No Hit
CGAGGCTA6932810.23358361505756084No Hit
GGCCTTCT5123550.1726251449164431TruSeq Adapter, Index 20 (100% over 8bp)
AATACGGA3105750.10464044340823123No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)