FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3024381
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT531631.7578142436419222No Hit
GTTCTTCCTAACGTAA163970.5421605280551623No Hit
GAAGCAGTGGAACCTT162020.5357129277032225No Hit
CATGGAGGATCGACAT126680.41886257055576No Hit
CGCTTAAGATAATAGG125780.41588675500871086No Hit
TGTCCCATGAGACAAT118900.39313829838237974No Hit
AAAGCATGATCTTTGT116110.38391327018652743No Hit
TGAGCGCACAGGACAC105500.34883171134853713No Hit
TGTCGAAACTTCGCAA101910.33696151377753003No Hit
TAATTGCCTGTCTGGC101750.336432479902499No Hit
GGAAGGACTAGGTAAT91770.30343399194744314No Hit
GATACGTACCGCTCGA87250.2884887849778186No Hit
GGGGGGGGGGGGGGGG86310.28538071096201173No Hit
CATGTCAGAGGTTTGT82800.27377503032852013No Hit
AATGATGGAAAGCTAA79930.2642854851951523No Hit
TCGACCTTGTTTCGCC74710.2470257550222674No Hit
GTGAGATCTCGATTAC68770.22738537241174311No Hit
GCTATGATGACCTGCG64400.21293613469996012No Hit
ATGGGTACTTGGCTGC63140.2087699929340913No Hit
AAACGACCTAGGGAGG59090.1953788229723702No Hit
CAGCTCACTGTTTACG58770.1943207552223083No Hit
TTGCGATGACATTCAG58100.19210542587061616No Hit
AGCCTACCTTAACTCG57560.1903199365423867No Hit
TGTTCCTCTTTATGGC56740.18760863793285304No Hit
CTGCCTTGACCATAAC53450.176730378877529No Hit
CAATGTGACCAACGCC52890.17487876031492064No Hit
AGCTTGTGACTCACTC50770.16786906147076044No Hit
TAACCAGACATCACAC50730.16773680300200272No Hit
CACAGCTCTGGACTGA49670.16423195357992262No Hit
CATGATGTGTCATGTG49000.16201662422823052No Hit
CCTGTTGACCCTAGTA48630.16079323339222143No Hit
ATGGGTACTTTCGGGC48600.16069403954065312No Hit
CATGCAAACGAAGGCT48430.16013194104843273No Hit
CTATTCAACCGAACGA47420.15679241471229982No Hit
ATGCCGCGATGCCACG47250.1562303162200794No Hit
TTGTTTGTGATACGCT46440.1535520822277352No Hit
ATTAGCGTGGTCGGTC44880.14839400194618335No Hit
CATTAGTCTATTCTGG44780.148063355774289No Hit
AGAACGCGAGGAATAT43880.14508754022723988No Hit
CACTATACTACGCCAC43770.1447238294381561No Hit
GCAGATACTCGGCTCA42530.1406238169066662No Hit
CCGCTATTGTTCGCAA41990.1388383275784367No Hit
GCATCTCTGAGCCTAT41220.13629235205485024No Hit
ATAGGAAACAGACTTC41200.13622622282047137No Hit
AGCTCGGACCTTAAGC40590.13420928117191586No Hit
AACCAACACCGGAATG40110.13262217954682298No Hit
CCCAATGCTGGAATGC40030.13235766260930748No Hit
AACGTCCCTTACAGAA39910.13196088720303428No Hit
TTGTAACCTCATCCTC39890.1318947579686554No Hit
CTGCCATTGCACAGTT39740.1313987887108139No Hit
TTGACGTGACCTCAAA39350.1301092686404259No Hit
GGGATCAGATGAGTAA39140.1294149116794478No Hit
CTGATTACTGCTTTGA38970.1288528131872274No Hit
GGGTCGTACCGCTCGA38960.12881974857003797No Hit
TGGAGTTACAAGTCTC38670.12786087467154436No Hit
TGGGCCATGCGTTCTC37810.12501731759325296No Hit
ATAGCCAACATGGCCC37730.12475280065573748No Hit
CCTACGGGATGGAACG37180.12293424671031858No Hit
CGATCAAACCGATCGC37090.12263666515561365No Hit
CGCTTACTGGCCACGT37060.12253747130404535No Hit
ATCTTACTGCGCACTG37040.12247134206966649No Hit
CGCTGAGTGTTAGCTG36980.12227295436652988No Hit
TCAGGCTCTTAAAGCA36790.12164472663993062No Hit
GATACCATGTCTGGAT36640.1211487573820891No Hit
TGTTTGTTGCGCTCGA36430.12045440042111097No Hit
CGCTTCCCTGCGCTGT36170.11959472037418567No Hit
CTCGGATCTTTCTGAG36170.11959472037418567No Hit
TCCAAAGTGAAGGCCA35850.11853665262412375No Hit
GTAAGAAACGACAAAT35350.11688342176465201No Hit
TTCTAACACTACGTTG35290.1166850340615154No Hit
CATGTTACTAACGTGG34980.11566003092864292No Hit
AGTGGTACTCAACGCC34580.11433744624106552No Hit
CGAGTCTCTTACTTTC34440.11387454160041344No Hit
TGGGTGCACTTCTGAG34100.11275034461597265No Hit
GCGTCGATGCTTGAAC33950.11225437535813114No Hit
CCTCCCTTGTTTCGCC33570.11099791990493262No Hit
AGAACAATGAGTCGGA33360.11030356294395448No Hit
AACAGCTACTCGAGAG33310.11013823985800732No Hit
ATTGAGAGATGATCAC33300.11010517524081788No Hit
TACGCCTTGGGTTGAG33260.10997291677206013No Hit
GCATATCGAGCCGCTA33240.10990678753768127No Hit
TACAACCGATTCCATT33090.10941081827983974No Hit
CTTAGGCCTTATGACC33000.10911323672513483No Hit
TGAGATAGACTCATCC32890.10874952593605104No Hit
CCTTCAGTGTAGCTTG32810.10848500899853555No Hit
TATCTCCCTGGAATTA32630.10788984588912574No Hit
GTGTACTGAATCACAC32270.10669951967030608No Hit
GCGACAAGAAAGGCTG32220.10653419658435892No Hit
CATGTCAGAACAGGTG32170.10636887349841173No Hit
GCATAGTACGCTCCTT32050.10597209809213852No Hit
ATCGCAATGTAACTCG32030.10590596885775966No Hit
AACCACTGAAAACATG31860.10534387036553927No Hit
TGGTCCTTGTTTCTGT31600.10448419031861395No Hit
GGTCAGAGAACCAGTT31370.10372370412325696No Hit
TTCACGCTGTGGGCAA31310.10352531642012035No Hit
ACCTCCATGGAATTGA30590.10114466398248104No Hit
CTAGAGACTCCGTTAA30530.10094627627934444No Hit
TTCTATGACCAACGCC30420.10058256549026065No Hit
CGGTTCACTGGAATGC30390.10048337163869234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)