FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16356026
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC15000.08.4001544
TATACTG18650.08.3325935
GTAGGAC15200.08.2895753
GAACCGT6300.08.2223736
GTGTAGG23600.08.1304741
TTAGGAC16650.07.98808573
TCTATAC14900.07.98667433
TATACAC29200.07.95900153
GTATAGA15850.07.9528091
TATACAG21150.07.9434085
CTAGGAC22150.07.9007743
ACCGTTC5500.07.8904518
TACACTG27700.07.8340795
TAGACAG32800.07.76843555
TAGGACA21100.07.7631764
TAGGACT22450.07.73288154
ACGAGAC12250.07.657142622
TCTAGAC18050.07.5236383
GTATTGA14550.07.50825361
CATACCG3208.5662396E-77.43763645
Produced by FastQC (version 0.11.5)