FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2523849
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT277901.1010959847439368No Hit
TGCTGCTACCGTTCTC254261.007429525300444No Hit
AAACGACCTCTTCAAG247680.9813582349815698No Hit
ATGGGTTACAATCGAA239840.9502945699207836No Hit
TATGCTTTGGACTGTT235420.9327816363023304No Hit
GCCTCTTGATCTGTGA226560.8976765250219012No Hit
AAAGCGGACTCGGGCT222380.8811145199257165No Hit
TCCAAAGTGCTCACGG208790.8272681923522367No Hit
GCAGGTCTGGCTGTTA198840.7878442806998358No Hit
TAGTGCATGAACCCGC196300.7777802871724893No Hit
TAACTCACTGGTGTCC187880.7444185448495533No Hit
TGTGGCTCTTACGATC187140.7414865152392239No Hit
GAAGTATGAACAGGTG183720.7279357837968912No Hit
TTCCCATCTCTTTGTT169990.6735347479187542No Hit
GAGCCTGACCGAGTTT165560.6559821922785396No Hit
TCACCTGACTCAATGC159470.6318523810259647No Hit
GGAACTACTACCAAGG159250.6309806965472181No Hit
TGAGAATGAGCAGTTT155260.6151715098644966No Hit
CTAGAGACTAATCTGC152350.6036415015319855No Hit
ATGTCCAGAGTCGGTC147180.5831569162814415No Hit
TTCGTCCGACCTGTGG145320.5757872202338571No Hit
TCGTATACTGCTAATC144120.5710325776225122No Hit
TCACACAGATTGGGAG141590.561008206116927No Hit
ATTCCTAACTGCTACA140350.5560950754185373No Hit
ACTGATTCTCGGCTCA136930.5425443439762045No Hit
TCCGCGAGAGAACAGC136900.542425477910921No Hit
AGGTCGAGAAGGGAGG135390.5364425526249788No Hit
TGGACCTCTAGGGAGG133560.5291917226426779No Hit
TGGCCTGGACCCGAGT131880.5225352229867952No Hit
CCATGATGATTCGGGC131380.5205541218987348No Hit
GCAATCATGGAACGTG130760.51809755654954No Hit
TGCGGTGACCTTAGTG115470.4575154852766548No Hit
TTCGATGGAAATCCTG115030.4557721163191617No Hit
ACGAGGATGGAGCTGA113450.4495118368808911No Hit
TCCCAGACTGTCTTGA108880.4314045729360195No Hit
TGGTCCTTGGAGAGGG107930.4276404808687049No Hit
AAGGTGCGAACAGATA107740.42688766245524196No Hit
TTTCGTAACAGTACGG106380.42149906749571786No Hit
TCTCTCTACTCTGGAT103670.4107614995984308No Hit
TCCTTCACTTGTCACA94050.3726451146641499No Hit
GAAGTAATGAGTCTGT93080.3688017785533128No Hit
TGATATCCTCGATGTT91450.36234338900623614No Hit
AGCCCAGTGAAAGTAC90750.3595698474829516No Hit
TTCGATGGACAGGTGA90570.3588566510912499No Hit
TCGCCGAGAAGGTAAT89780.3557265113721146No Hit
AGCTGCTCTTGTCACA88930.352358639522412No Hit
CTTTCGGTGCTTTGTT88530.35077375865196375No Hit
AACTCCCTGCTTCACC86840.3440776369743198No Hit
AACCCTCACGCGGTTC85450.33857017594951205No Hit
ATGGCCGTGATACGCT83410.3304872835102258No Hit
CCCTTGCCTCCTACGG82370.32636659324706035No Hit
TGTTTGTTGCAAGTTC77730.30798197514986037No Hit
TCGGCTTACCTCCTAC77610.30750651088872594No Hit
AAACACGCTGCAGTTT77200.30588200799651644No Hit
ACCCTTGTGGGCCATC72930.2889634047044812No Hit
GGGGGGGGGGGGGGGG72710.28809172022573454No Hit
CTAACTGGAACCTTGA72510.28729927979051045No Hit
TGGCCTTTGCGAAAGC72410.28690305957289836No Hit
CAATGTGTGGTTGACT71150.28191068483098636No Hit
TCAAAGGCTCTTAGTG70910.2809597563087174No Hit
TACAACGTGAGCAGGT70240.2783050808507165No Hit
AAATAGGACTCTATCT69550.27557116134919324No Hit
GGACATTTGGCCGCTA69090.27374854834817774No Hit
ACAGCCGACAAACATG67980.2693505039326838No Hit
CTTTCGGTGAGCCATT67010.26550716782184675No Hit
TGAACTGTGTGCTGGC64090.2539375374675743No Hit
CAGCCGATGTAATAGG62160.24629048726766142No Hit
GGCTTGCGAATACGCT61270.242764127330914No Hit
AGTGTTCGATTCGCCT61050.24189244285216746No Hit
GATAAGAACATTGGTC56220.22275500634150458No Hit
TTGGGAGGAATCTGTG53660.21261176877063565No Hit
GTGCTAATGGGAGTCA53200.21078915576962015No Hit
GTAAAGCCTGAAATCA52880.2095212510732615No Hit
TGTACTGGAAATGCTC51740.20500434059248393No Hit
CGACGTTCTTGCTACA51360.20349870376555806No Hit
GGAACATCTTTACGAC50590.20044780808994517No Hit
GGACCACTGGCTAGAG49020.19422715067343568No Hit
AGGGACCCTAATCCTG48370.19165171925895724No Hit
GGGTGAAACCGACTAT47280.1873329188869857No Hit
TCTTGAACTCGTACAT46890.18578766003829864No Hit
TACGTGTGAAACGCAG46560.18448013332017882No Hit
TACCACCTGAAACATG45690.18103301742695382No Hit
TAACGAGCTTACTTTC45350.1796858686870728No Hit
CGGATGAGAACAGGCA44960.17814060983838573No Hit
AGCTTGTGAACCGTGC44840.17766514557725127No Hit
ACTTATCTGTCAGGTT43590.1727123928571004No Hit
CATGATGTGCCTAGTA43450.1721576845524435No Hit
AACTAGCACTGCCACG41740.16538231883127713No Hit
GTCCGAGTGGTCTTGA40130.15900317332772287No Hit
AACTACGACATTGCCA39650.15710131628318494No Hit
ACGTGTTGATCTTTGT39110.15496172710807976No Hit
TCGGACCGAATGTCCA38890.15409004262933323No Hit
GTAGATAGAGCTGAGA38570.15282213793297458No Hit
TGGTAGGGATGTGCTA38310.1517919653671832No Hit
GGTAGTTTGTCCAGCG37750.14957313214855564No Hit
AACGAACCTGACACCC37750.14957313214855564No Hit
CTCGGATCTAGTACGG37430.148305227452197No Hit
CGTCGTACTTGCGCCA37270.1476712751040177No Hit
TTTAGGGACTGGGCAA36800.145809040081241No Hit
CCTTCAGTGCAACGCC36340.1439864270802255No Hit
TACCACCTGTACTTTC36100.14303549855795653No Hit
AGTTTGCGAGCAGGAT35850.14204494801392634No Hit
GCCATAATGACCGTTA35720.14152986173103066No Hit
TGAGGAGTGGTCCCAG35430.14038082309995567No Hit
CTTTAACTGTTCGCCT34820.13796387977252203No Hit
CAGGCCATGTCGTAGT33210.13158473426896777No Hit
GAGGTTGGAGGAATAT33180.13146586820368414No Hit
AGAACGCGACACAGTT32540.1289300588109669No Hit
CTTACATTGGAAGGCT32350.12817724039750397No Hit
GTCCGTTACGGATGGT32350.12817724039750397No Hit
GGGATGTGATGCATGT32210.12762253209284707No Hit
GAGAAGAGATGCTACA31970.1266716035705781No Hit
GAAAGTCCTTATCTGG31880.12631500537472726No Hit
CGATCGGTGCGAGGAT31620.12528483280893588No Hit
GAACCATACTTTGGGT31220.1236999519384876No Hit
TGCAATGACTCCGTAA31070.1231056216120695No Hit
CCGTTACTGTTGGTTG30660.12148111871986003No Hit
CAAAGACCTGCTTTGA30650.12144149669809881No Hit
AACTACGACGCTGTTA29730.1177962706960678No Hit
GGCTTAAACTGCATCA29650.11747929452197814No Hit
GCTGGACACATTGGTC29270.11597365769505227No Hit
AATTCTGCTTAGCGAG29040.11506235119454451No Hit
TCCTCATCTTTCTGGA28540.11308125010648419No Hit
TCGTAGAACCGCACTG27970.11082279486609539No Hit
TGTCTTCCTGAAATCA27920.11062468475728936No Hit
CCCACCTCTTGACCTA27650.10955489016973678No Hit
ATGGCCGTGGGCCAAA27490.10892093782155748No Hit
CCTGAGGACTGACTCA27320.10824736345161695No Hit
ACAAGACCTAAGCTAA26990.10693983673349713No Hit
TATGGGTACCCTTTGC26810.10622664034179541No Hit
TCCGTAAGATTACGCA26570.10527571181952644No Hit
ATAGCCAACATCTGTG26330.10432478329725747No Hit
AGGTGATCTGTTAGTC25970.10289839051385404No Hit
ATCTCTTGACGGTACC25350.10044182516465922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)