FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623112
Sequences flagged as poor quality0
Sequence length8
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACAGGG250905095.65165345589513No Hit
GGCCAGGG340641.2986102004031852ABI Solid3 GAPDH Forward Primer (100% over 8bp)
GGACAGGA302691.1539347157117195No Hit
GGACAGGC53830.20521426458344136No Hit
GGACACGG47930.18272189673944536No Hit
GGAAAGGG40260.15348181854225057No Hit
GGACAGCG36430.13888084077233454No Hit
CGACAGGG34480.13144692258660706No Hit
GCACAGGG32610.12431798565978121No Hit
GGTCAGGG28160.10735340313337745No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)