FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31309798
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC31450.011.03953722
CGAGACA34350.09.94451423
ATCTCCG36250.09.8479810
CTTATAC41900.09.5950481
CACGAGA38000.09.24719321
TCTCCGA39250.08.98825111
CCCACGA39800.08.86401319
TAGACTG44000.08.5587925
TACACCG14700.08.3806595
TAGACAG57650.08.3535685
TATACAC67150.08.3183863
GTATAGG27250.08.224941
CCACGAG45100.08.10175120
GTGTAGG49600.08.049011
CTAGGAC48050.07.98305943
TATACTG39050.07.9587495
GTATAGA33100.07.8716221
CCTATAC24550.07.8123423
TCTATAC29100.07.60109763
TTATACA62000.07.4739212
Produced by FastQC (version 0.11.5)