FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29973742
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG406090.13548191613846547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG35600.08.3740435
TACACTG55600.08.3573565
GTGTAGG50050.08.1060751
TAGACTG37850.08.0611355
TAGACAG51400.08.0327555
GTGTATA54400.07.8693571
CTAGGAC44500.07.7653223
CTAGTAC18450.07.6585683
TAGTACT27150.07.57608564
GTCTAAG31600.07.48197751
TCTACAC41350.07.4433723
TACACCG13550.07.43701845
GTGTAAG44800.07.36971571
TAGGACT41100.07.2856674
TCTAGAC32800.07.25099473
TAGGACC29950.07.2415624
CTTAGAC31500.07.23935033
AGGACCG28650.07.23007355
TCTATAC27650.07.1848183
TGTACTG44050.07.18072275
Produced by FastQC (version 0.11.5)