FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004946589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843145
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA81283796.40536325305848No Hit
GGCAGGGA59500.7056911919064929No Hit
GGCAAGGC44060.5225672926957996No Hit
GGCAAGAA25910.30730182827390307No Hit
GGCAACGA22040.2614022499095648No Hit
GGCAAGCA18440.21870496771018033Clontech Universal Primer Mix Long (100% over 8bp)
CGCAAGGA15710.18632619537564712No Hit
GCCAAGGA14920.17695651400411555RNA PCR Primer, Index 1 (100% over 8bp)
GGAAAGGA11880.14090103125796868No Hit
GGCCAGGA10300.12216166851490551No Hit
GGCAATGA9230.10947108741675513No Hit
GGCAAGTA8830.10472694495015686No Hit
GTCAAGGA8690.10306649508684745No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)