FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004946592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7456639
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA722351896.87364508326071No Hit
GTGGCGCA717220.9618542616854591No Hit
GTGGAGCC183760.24643810703455002No Hit
GGGGAGCA143470.19240572059342015No Hit
GAGGAGCA125840.16876236062923256No Hit
GTGGAGCT125130.16781018901411213No Hit
GTGGACCA100690.13503402806545953ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)
GTGCAGCA96700.12968309180583906No Hit
GTGGAGAA90690.1216231602468619No Hit
GTGTAGCA87400.11721098473454328No Hit
CTGGAGCA83330.11175276153237404No Hit
GTGGAGGA77660.10414879947922917No Hit
GTGGTGCA77330.10370624084121546No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)