FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004946597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825048
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA79422196.26361132928024No Hit
GGCAGGGA59740.7240790838836043No Hit
GGCAAGGC45090.5465136573872066No Hit
GGCAAGAA26220.3177996916543037No Hit
GGCAACGA21830.2645906662400248No Hit
GGCAAGCA18300.2218052767838962Clontech Universal Primer Mix Long (100% over 8bp)
CGCAAGGA15290.18532255093037012No Hit
GCCAAGGA13480.16338443339054212RNA PCR Primer, Index 1 (100% over 8bp)
GGAAAGGA12880.15611212923369305No Hit
GGCCAGGA10520.1275077328834201No Hit
GGCAATGA9810.11890217296448206No Hit
GGGAAGGA9720.11781132734095472No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)