FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004946623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3743646
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT400791.0705873365163265No Hit
TGCTGCTACCGTTCTC385401.0294776803148589No Hit
AAACGACCTCTTCAAG380561.016549107474371No Hit
ATGGGTTACAATCGAA368100.9832660459883227No Hit
TATGCTTTGGACTGTT364120.9726346989004837No Hit
GCAGGTCTGGCTGTTA338570.9043857245049345No Hit
AAAGCGGACTCGGGCT336610.8991501867430841No Hit
TCCAAAGTGCTCACGG323400.8638637307053072No Hit
TAGTGCATGAACCCGC298290.7967900811134385No Hit
GAAGTATGAACAGGTG298190.7965229618398748No Hit
TAACTCACTGGTGTCC280960.7504983110048332No Hit
TGTGGCTCTTACGATC279200.7457970117901105No Hit
TTCCCATCTCTTTGTT253120.6761323052446733No Hit
TCACCTGACTCAATGC242070.6466156255158741No Hit
GGAACTACTACCAAGG238470.6369993316675775No Hit
GCCTCTTGATCTGTGA237030.633152814128259No Hit
TGAGAATGAGCAGTTT235440.6289056176785947No Hit
GAAGTAATGAGTCTGT227740.6083374336141825No Hit
CTAGAGACTAATCTGC221580.5918828863626528No Hit
TTCGTCCGACCTGTGG218890.5846973779037868No Hit
TCGTATACTGCTAATC218560.5838158843010264No Hit
ATGTCCAGAGTCGGTC218170.5827741191341276No Hit
TCACACAGATTGGGAG218140.5826939833520584No Hit
ATTCCTAACTGCTACA215150.574707117072501No Hit
TGCGGTGACCTTAGTG214920.5740927427433042No Hit
AGGTCGAGAAGGGAGG211740.5655983498439756No Hit
CCATGATGATTCGGGC208790.5577183312738437No Hit
TCCGCGAGAGAACAGC208730.5575580597097054No Hit
ACTGATTCTCGGCTCA205000.547594510805776No Hit
TGGACCTCTAGGGAGG201630.5385925912866761No Hit
GCAATCATGGAACGTG193630.5172230494015727No Hit
TGGCCTGGACCCGAGT186150.4972425277390009No Hit
TTCGATGGAAATCCTG175980.4700764976175632No Hit
ACGAGGATGGAGCTGA169710.45332811916511334No Hit
TTTCGTAACAGTACGG168980.45137814846809765No Hit
AAGGTGCGAACAGATA168710.45065692642947547No Hit
TCCCAGACTGTCTTGA161150.43046270934805264No Hit
TGGTCCTTGGAGAGGG160320.4282456193774732No Hit
TCTCTCTACTCTGGAT158860.4243456779834418No Hit
GCGGTTCGAGTACGTT154260.41205819139950733No Hit
TCCTTCACTTGTCACA140900.3763710564513846No Hit
TGATATCCTCGATGTT138260.3693191076293004No Hit
TCGCCGAGAAGGTAAT137780.36803693511619423No Hit
TTCGATGGACAGGTGA137600.3675561204237794No Hit
AGCTGCTCTTGTCACA136820.3654725900899818No Hit
CTTTCGGTGCTTTGTT132900.3550015145662811No Hit
AACTCCCTGCTTCACC131270.35064747040719124No Hit
AGCCCAGTGAAAGTAC125740.3358757745791135No Hit
TCGGCTTACCTCCTAC121700.32508415592713624No Hit
AACCCTCACGCGGTTC121410.32430951003380126No Hit
CCCTTGCCTCCTACGG120200.3210773668236794No Hit
ATGGCCGTGATACGCT118860.31749796855792456No Hit
TGTTTGTTGCAAGTTC116570.3113809371933137No Hit
AAACACGCTGCAGTTT116310.3106864270820478No Hit
GGGGGGGGGGGGGGGG112800.3013105405799587No Hit
CTAACTGGAACCTTGA108590.290064819162923No Hit
TACAACGTGAGCAGGT107120.28613816584153523No Hit
CAATGTGTGGTTGACT106850.285416943802913No Hit
TGGCCTTTGCGAAAGC105950.28301287034083883No Hit
TCAAAGGCTCTTAGTG105140.2808492042249721No Hit
AAATAGGACTCTATCT103740.277109534395079No Hit
ACAGCCGACAAACATG102050.27259521867185094No Hit
GGACATTTGGCCGCTA101150.2701911452097768No Hit
CTTTCGGTGAGCCATT100530.2685350057136813No Hit
TGAACTGTGTGCTGGC98730.263726858789533No Hit
ACCCTTGTGGGCCATC98230.262391262421714No Hit
CAGCCGATGTAATAGG91860.2453757646957004No Hit
AGTGTTCGATTCGCCT91570.2446011188023654No Hit
GAGGTTGGAGGAATAT90870.24273128388741885No Hit
GATAAGAACATTGGTC86290.23049722115819712No Hit
GGCTTGCGAATACGCT85350.22798629998669745No Hit
GTGCTAATGGGAGTCA82610.22066723189104956No Hit
CGACGTTCTTGCTACA79910.2134550115048271No Hit
GTAAAGCCTGAAATCA79680.2128406371756304No Hit
TTGGGAGGAATCTGTG78850.21062354720505091No Hit
TGTACTGGAAATGCTC76200.2035448864556104No Hit
GGAACATCTTTACGAC74420.19879016338617486No Hit
GGGTGAAACCGACTAT70870.1893074291746602No Hit
TACCACCTGAAACATG70790.18909373375580918No Hit
AGGGACCCTAATCCTG70370.18797183280684127No Hit
TCTTGAACTCGTACAT69780.18639582909281488No Hit
CGGATGAGAACAGGCA69190.1848198253787885No Hit
TAACGAGCTTACTTTC68910.18407189141280986No Hit
AGCTTGTGAACCGTGC68560.18313697395533657No Hit
TACGTGTGAAACGCAG68490.18294999046384194No Hit
ACTTATCTGTCAGGTT66120.17661926368038006No Hit
CATGATGTGCCTAGTA65540.17506997189371No Hit
AACTAGCACTGCCACG63210.16884609281967367No Hit
CGTCGTACTTGCGCCA59360.15856200078746763No Hit
TGGTAGGGATGTGCTA58850.15719969249229226No Hit
ACGTGTTGATCTTTGT58640.1566387420178083No Hit
AACTACGACATTGCCA57380.1532730391709045No Hit
TCGGACCGAATGTCCA57360.15321961531619177No Hit
GTAGATAGAGCTGAGA56230.15020116752492088No Hit
TTTAGGGACTGGGCAA55970.14950665741365504No Hit
AACGAACCTGACACCC55800.14905255464859657No Hit
GGTAGTTTGTCCAGCG55500.1482511968279052No Hit
CCTTCAGTGCAACGCC55330.14779709406284675No Hit
CTCGGATCTAGTACGG55250.14758339864399572No Hit
AGTTTGCGAGCAGGAT54820.1464347857676714No Hit
TACCACCTGTACTTTC54230.14485878205364502No Hit
TGAGGAGTGGTCCCAG53540.14301565906605487No Hit
CTTTAACTGTTCGCCT50610.1351890643506357No Hit
AGAACGCGACACAGTT49210.1314493945207426No Hit
CAGGCCATGTCGTAGT48980.1308350201915459No Hit
GGGATGTGATGCATGT48660.12998023851614177No Hit
GAGAAGAGATGCTACA48530.1296329834605088No Hit
CTTACATTGGAAGGCT47910.1279768439644133No Hit
GAAAGTCCTTATCTGG46850.1251453796646371No Hit
CGATCGGTGCGAGGAT46830.12509195580992433No Hit
GCCATAATGACCGTTA46480.12415703835245105No Hit
TGCAATGACTCCGTAA46250.12354266402325434No Hit
CAAAGACCTGCTTTGA45650.12193994838187157No Hit
GAAGGACACCCAGCTG45340.12111187863382382No Hit
TCCTCATCTTTCTGGA45210.12076462357819089No Hit
AACTACGACGCTGTTA44820.11972285841129209No Hit
GCTGGACACATTGGTC44700.11940231528301554No Hit
AATTCTGCTTAGCGAG44440.11870780517174968No Hit
CCGTTACTGTTGGTTG43410.1159564766540426No Hit
GGCTTAAACTGCATCA42830.11440718486737261No Hit
TCGTAGAACCGCACTG41460.11074765081954864No Hit
CCCACCTCTTGACCTA41120.10983944528943175No Hit
TGTCTTCCTGAAATCA41080.10973259758000622No Hit
ATAGCCAACATCTGTG41060.10967917372529347No Hit
GGACCACTGGCTAGAG40950.10938534252437329No Hit
ATGGCCGTGGGCCAAA40700.10871754434046382No Hit
TCCGTAAGATTACGCA40670.10863740855839468No Hit
CCTGAGGACTGACTCA40650.10858398470368193No Hit
AGGTGATCTGTTAGTC40150.10724838833586295No Hit
GCGTTTAGAAGTCTAC39690.10601963967746951No Hit
ACAAGACCTAAGCTAA39660.10593950389540037No Hit
TATGGGTACCCTTTGC38730.10345529465125708No Hit
GCGTTAATGGTACGTT37540.10027657529584795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)