FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5403352
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT517934495.8542771227934No Hit
CTTTCCTA981491.8164465317084655No Hit
CTTTCCAT150860.2791970613796769No Hit
CTTTACTT144670.26774120953067654No Hit
CTTTCATT119340.22086290139898346No Hit
CTATCCTT102340.18940094963274648No Hit
ATTTCCTT99620.1843670373501486No Hit
CATTCCTT98740.18273841867048454TruSeq Adapter, Index 27 (100% over 8bp)
CGTTCCTT77420.14328142974953326No Hit
CTTTCCGT75790.14026478378606463No Hit
CTTACCTT75070.13893227759361226No Hit
CTGTCCTT55010.10180717450945266No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)