FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735670
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT480041.7547438104742166No Hit
GAAGCAGTGGAACCTT156250.5711580709661619No Hit
GTTCTTCCTAACGTAA140860.5149012856082787No Hit
GATACGTACCGCTCGA137810.5037522800630193No Hit
CGCTTAAGATAATAGG111860.40889434763695914No Hit
CATGGAGGATCGACAT109290.3994999396857077No Hit
TGTCCCATGAGACAAT107270.3921160081442571No Hit
AAAGCATGATCTTTGT99630.3641886631062957No Hit
TGAGCGCACAGGACAC95290.3483241765271396No Hit
GGGGGGGGGGGGGGGG92630.3386007815270117No Hit
TGTCGAAACTTCGCAA92380.3376869286134658No Hit
TAATTGCCTGTCTGGC90310.3301202264893061No Hit
GGAAGGACTAGGTAAT76120.27824993511644314No Hit
CATGTCAGAGGTTTGT72580.2653097778606338No Hit
AATGATGGAAAGCTAA72560.2652366696275501No Hit
TCGACCTTGTTTCGCC68740.2512729971085694No Hit
GCTATGATGACCTGCG55820.2040450785365194No Hit
ATGGGTACTTGGCTGC55380.20243669740867865No Hit
CAGCTCACTGTTTACG52030.19019106836716415No Hit
AAACGACCTAGGGAGG51950.1898986354348295No Hit
AGCCTACCTTAACTCG50760.1855486955663512No Hit
TTGCGATGACATTCAG50160.18335544857384115No Hit
TGTTCCTCTTTATGGC49470.18083321453245457No Hit
CAATGTGACCAACGCC46730.17081738659999196No Hit
CCTGTTGACCCTAGTA46030.16825859844206356No Hit
GTGAGATCTCGATTAC45740.16719852906235036No Hit
CTGCCTTGACCATAAC45600.16668677143076466No Hit
AGCTTGTGACTCACTC44850.1639452126901271No Hit
CATGCAAACGAAGGCT44580.16295825154349758No Hit
TAACCAGACATCACAC44200.16156919511490786No Hit
CACAGCTCTGGACTGA43900.16047257161865283No Hit
ATGGGTACTTTCGGGC43540.1591566234231468No Hit
CATGATGTGTCATGTG43330.15838898697576828No Hit
CTATTCAACCGAACGA42880.15674405173138573No Hit
TGGGCCATGCGTTCTC41350.15115127190048508No Hit
AGAACGCGAGGAATAT40860.1493601201899352No Hit
ATGCCGCGATGCCACG40820.14921390372376786No Hit
TTGTTTGTGATACGCT40460.14789795552826182No Hit
CATTAGTCTATTCTGG39580.1446811932725804No Hit
ATTAGCGTGGTCGGTC39270.14354801565978353No Hit
GCATCTCTGAGCCTAT39120.142999703911656No Hit
GGGCTGACTAATACAG38950.14237828393044483No Hit
GGGTCGTACCGCTCGA38940.142341729813903No Hit
CCGCTATTGTTCGCAA38300.1400022663552256No Hit
CACTATACTACGCCAC37620.13751658643038087No Hit
GGCTTGCGAGTACGTT36470.1333128630280699No Hit
GCAGATACTCGGCTCA36200.1323259018814404No Hit
AGCTCGGACCTTAAGC35890.1311927242686435No Hit
AACCAACACCGGAATG35860.131083061919018No Hit
TTGACGTGACCTCAAA35760.13071752075359966No Hit
ATAGGAAACAGACTTC35440.12954778902426095No Hit
GGTAGCCTGTGCCACG35410.12943812667463547No Hit
AACGTCCCTTACAGAA35210.12870704434379876No Hit
CCCAATGCTGGAATGC35120.12837805729492227No Hit
CTGCCATTGCACAGTT35100.12830494906183862No Hit
TGGAGTTACAAGTCTC34290.12534406562195002No Hit
TTGTAACCTCATCCTC33960.12413777977606949No Hit
GGGATCAGATGAGTAA33780.12347980567831646No Hit
CTGATTACTGCTTTGA33740.12333358921214911No Hit
GATACCATGTCTGGAT33740.12333358921214911No Hit
CGATCAAACCGATCGC33690.12315081862943995No Hit
CCTACGGGATGGAACG33610.12285838569710528No Hit
TGTTTGTTGCGCTCGA33170.12125000456926456No Hit
ATAGCCAACATGGCCC33040.12077480105422071No Hit
CATGTTACTAACGTGG32450.1186181081782525No Hit
CGCTTACTGGCCACGT32440.11858155406171067No Hit
ATCTTACTGCGCACTG32380.11836222936245964No Hit
CGCTGAGTGTTAGCTG32110.11737526821583014No Hit
CGCTTCCCTGCGCTGT31830.11635175295265877No Hit
TCAGGCTCTTAAAGCA31650.11569377885490574No Hit
TGGGTGCACTTCTGAG31490.11510891299023639No Hit
TCCAAAGTGAAGGCCA30950.11313499069697734No Hit
TTCTAACACTACGTTG30760.1124404624826825No Hit
CTCGGATCTTTCTGAG30360.11097829782100913No Hit
TACAACCGATTCCATT30160.11024721549017243No Hit
AGCCGGTACGCGCAAA30110.11006444490746325No Hit
CGAGTCTCTTACTTTC30040.10980856609167042No Hit
CCTCCCTTGTTTCGCC29840.10907748376083372No Hit
CTTAGGCCTTATGACC29720.10863883436233171No Hit
ATTGAGAGATGATCAC29500.10783464379841136No Hit
AACAGCTACTCGAGAG29410.10750565674953484No Hit
TACGCCTTGGGTTGAG29370.1073594402833675No Hit
AGTGGTACTCAACGCC29330.10721322381720018No Hit
GCATAGTACGCTCCTT29250.10692079088486549No Hit
GTAAGAAACGACAAAT29230.10684768265178182No Hit
CATGTCAGAACAGGTG29200.10673802030215633No Hit
ACCTGCTTGCCAAACT29150.10655524971944716No Hit
GTCCTGACTTTACGAC29080.10629937090365432No Hit
TGAGATAGACTCATCC28930.10575105915552681No Hit
AGAACAATGAGTCGGA28830.10538551799010845No Hit
AACCACTGAAAACATG28710.10494686859160644No Hit
ACCTCCATGGAATTGA28640.1046909897758136No Hit
GCATGGGCTATGTGGG28580.10447166507656261No Hit
CCTTCAGTGTAGCTTG28550.10436200272693709No Hit
ATCGCAATGTAACTCG28500.10417923214422792No Hit
GCATATCGAGCCGCTA28360.10366747451264224No Hit
TGGTCCTTGTTTCTGT28260.1033019333472239No Hit
GAAAGTAACCCTGCGA28110.10275362159909637No Hit
TATCTCCCTGGAATTA28100.10271706748255455No Hit
GTGTACTGAATCACAC28090.10268051336601272No Hit
TTCTATGACCAACGCC27960.10220530985096887No Hit
GTTGCTAGACGCTCGA27700.10125490282088118No Hit
TTCACGCTGTGGGCAA27580.10081625342237917No Hit
CCTTCTTTGGATCATC27410.10019483344116797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)