FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504792
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT437551.7468516347864413No Hit
GAAGCAGTGGAACCTT165940.6624901389017531No Hit
GTTCTTCCTAACGTAA131510.5250336155656837No Hit
GATACGTACCGCTCGA121510.4851101408819575No Hit
CGCTTAAGATAATAGG101540.4053829619385562No Hit
TGTCCCATGAGACAAT100500.40123092057144866No Hit
CATGGAGGATCGACAT99200.39604086886256423No Hit
TGAGCGCACAGGACAC93010.3713282380333377No Hit
AAAGCATGATCTTTGT91790.3664575741219231No Hit
TGTCGAAACTTCGCAA83450.3331613962356954No Hit
TAATTGCCTGTCTGGC83420.33304162581164426No Hit
GGAAGGACTAGGTAAT77690.3101654748178691No Hit
GGGGGGGGGGGGGGGG68400.27307656683668746No Hit
AATGATGGAAAGCTAA65090.25986189671637405No Hit
CATGTCAGAGGTTTGT64880.2590235037480158No Hit
TCGACCTTGTTTCGCC61820.24680692049479555No Hit
GTGAGATCTCGATTAC55480.22149543754531315No Hit
ATGGGTACTTGGCTGC50610.20205270537433845No Hit
AAACGACCTAGGGAGG49010.19566494942494225No Hit
GCTATGATGACCTGCG48910.195265714678105No Hit
CAGCTCACTGTTTACG46430.1853646929565409No Hit
AGCCTACCTTAACTCG46270.1847259173616013No Hit
TTGCGATGACATTCAG46130.18416698871602913No Hit
TGTTCCTCTTTATGGC44920.17933624827929823No Hit
CCTGTTGACCCTAGTA43100.17207017588686005No Hit
CAATGTGACCAACGCC42670.17035346647545985No Hit
TAACCAGACATCACAC41750.166680506804557No Hit
CTGCCTTGACCATAAC41000.16368624620327754No Hit
AGCTTGTGACTCACTC40260.1607319090766818No Hit
CATGCAAACGAAGGCT39810.15893535271591414No Hit
ATGGGTACTTTCGGGC39450.1574981076273No Hit
CACAGCTCTGGACTGA39350.15709887288046273No Hit
ATGCCGCGATGCCACG39180.15642017381083936No Hit
CATGATGTGTCATGTG38990.15566162779184858No Hit
ATTAGCGTGGTCGGTC38160.1523479793930993No Hit
CTATTCAACCGAACGA37770.150790963880434No Hit
TGGGCCATGCGTTCTC37450.14951341269055474No Hit
AGAACGCGAGGAATAT36750.1467187694626939No Hit
CATTAGTCTATTCTGG36460.14556098869686585No Hit
TTGTTTGTGATACGCT36340.14508190700066112No Hit
CACTATACTACGCCAC35990.1436845853867307No Hit
CCGCTATTGTTCGCAA35560.1419678759753305No Hit
GGGTCGTACCGCTCGA35240.14069032478545124No Hit
GGGCTGACTAATACAG35020.13981200834240926No Hit
CCCAATGCTGGAATGC33330.13306494112085954No Hit
ATAGGAAACAGACTTC33200.1325459359499711No Hit
GGCTTGCGAGTACGTT32670.1304299917917336No Hit
CCTACGGGATGGAACG32660.13039006831704988No Hit
AACCAACACCGGAATG32650.13035014484236615No Hit
TTGACGTGACCTCAAA32550.1299509100955289No Hit
GCAGATACTCGGCTCA32490.12971136924742654No Hit
AGCTCGGACCTTAAGC32450.12955167534869164No Hit
GGTAGCCTGTGCCACG32300.12895282322843574No Hit
AACGTCCCTTACAGAA32120.12823420068412866No Hit
TGGAGTTACAAGTCTC31970.12763534856387276No Hit
CTGCCATTGCACAGTT31780.12687680254488196No Hit
ATAGCCAACATGGCCC31560.12599848610183997No Hit
CTGATTACTGCTTTGA31430.12547948093095154No Hit
TTGTAACCTCATCCTC31350.12516009313348175No Hit
GATACCATGTCTGGAT31220.1246410879625933No Hit
TCAGGCTCTTAAAGCA30820.12304414897524425No Hit
CGATCAAACCGATCGC30540.12192629168409992No Hit
CGCTGAGTGTTAGCTG30270.12084835786763931No Hit
CGCTTACTGGCCACGT30130.12028942922206715No Hit
CTCGGATCTTTCTGAG30100.12016965879801597No Hit
TGTTTGTTGCGCTCGA29960.11961073015244379No Hit
ATCTTACTGCGCACTG29850.11917157193092281No Hit
GCGTCGATGCTTGAAC29770.118852184133453No Hit
TCCAAAGTGAAGGCCA29510.11781417379167611No Hit
TTCTAACACTACGTTG29260.11681608692458295No Hit
TGGGTGCACTTCTGAG28850.11517922446255019No Hit
CGCTTCCCTGCGCTGT28560.11402144369672211No Hit
CATGTTACTAACGTGG28400.11338266810178252No Hit
CGAGTCTCTTACTTTC28320.11306328030431269No Hit
CTTAGGCCTTATGACC27980.11170588216506601No Hit
ACCTGCTTGCCAAACT27900.11138649436759618No Hit
GCGACGTTGCTTAGTG27890.11134657089291247No Hit
AGTGGTACTCAACGCC27870.111266723943545No Hit
GTAAGAAACGACAAAT27340.10915077978530753No Hit
TACGCCTTGGGTTGAG27190.10855192766505163No Hit
AGAACAATGAGTCGGA27180.10851200419036791No Hit
AGCCGGTACGCGCAAA27160.10843215724100044No Hit
CCTCCCTTGTTTCGCC27070.10807284596884692No Hit
GAAAGTAACCCTGCGA26920.10747399384859102No Hit
CCTTCAGTGTAGCTTG26850.10719452952580492No Hit
ATCGCAATGTAACTCG26810.10703483562707003No Hit
TACAACCGATTCCATT26710.10663560088023276No Hit
TGAGATAGACTCATCC26480.10571736096250707No Hit
GTCCTGACTTTACGAC26410.10543789663972097No Hit
AACAGCTACTCGAGAG26250.10479912104478135No Hit
TATCTCCCTGGAATTA26220.10467935062073018No Hit
AACCACTGAAAACATG26210.10463942714604646No Hit
ATTGAGAGATGATCAC26180.10451965672199529No Hit
GTGTACTGAATCACAC26040.10396072807642312No Hit
TTCACGCTGTGGGCAA25990.10376111070300449No Hit
GTTGCTAGACGCTCGA25920.1034816463802184No Hit
TGGTCCTTGTTTCTGT25850.10320218205743231No Hit
GCGACAAGAAAGGCTG25660.1024436360384415No Hit
AGAGAATTGCCCGCAA25280.10092654400045992No Hit
GGTCAGAGAACCAGTT25270.1008866205257762No Hit
CGGTTCACTGGAATGC25190.1005672327283064No Hit
AAAGGATACTGCTGGC25150.10040753882957147No Hit
TCCGGGATGCGAGTTT25140.10036761535488777No Hit
TTCTATGACCAACGCC25080.1001280745067854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)