FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3401596
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGTCAA327906196.39772036420551No Hit
TTAGTCAA713142.0964864728204056No Hit
TCAGACAA82100.24135729228279904No Hit
TCAGGCAA73840.21707457322974275No Hit
TCAGTAAA65270.19188051726307298No Hit
ACAGTCAA58850.17300702376178712TruSeq Adapter, Index 13 (100% over 8bp)
TCAGTCAT36150.10627364331331528No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)