FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504792
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATC245470598.0003529235162Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGATA109100.4355651087994532No Hit
ATATGATC65450.2612991418049882No Hit
ATCTGCTC55980.22349161127949946No Hit
ATCAGATC48800.19482655645658403Illumina PCR Primer Index 9 (100% over 8bp)
AACTGATC35410.1413690238550746No Hit
ATCTGAAC34810.13897361537405103No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)