FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533256
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCCT247582797.73299658621158No Hit
TCACTCCA148940.5879390002431653No Hit
ACACTCCT76580.3022987017498429No Hit
TCCCTCCT40270.1589653789431467No Hit
GCACTCCT40030.15801798160154362No Hit
TCACACCT35500.14013585677878587No Hit
TCACTCAT34580.13650416696930748RNA PCR Primer, Index 45 (100% over 8bp)
TCACTACT25820.10192416400079582No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)