FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533256
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT451481.7822122991122888No Hit
GAAGCAGTGGAACCTT160890.635111492877151No Hit
GTTCTTCCTAACGTAA131320.5183842454138073No Hit
CGCTTAAGATAATAGG107030.42249973946573105No Hit
CATGGAGGATCGACAT103640.4091177520155878No Hit
GATACGTACCGCTCGA102930.40631503488001214No Hit
TGTCCCATGAGACAAT100760.3977489839163511No Hit
AAAGCATGATCTTTGT96750.38191955333373334No Hit
TGAGCGCACAGGACAC93280.36822176676972246No Hit
TAATTGCCTGTCTGGC86790.34260256365720637No Hit
TGTCGAAACTTCGCAA86010.33952352229699645No Hit
GGAAGGACTAGGTAAT76130.3005223317343372No Hit
GGGGGGGGGGGGGGGG68480.27032404147073963No Hit
AATGATGGAAAGCTAA68030.2685476714552339No Hit
CATGTCAGAGGTTTGT67760.26748184944593045No Hit
TCGACCTTGTTTCGCC63530.25078397130017654No Hit
GTGAGATCTCGATTAC56850.22441474529222472No Hit
ATGGGTACTTGGCTGC53050.20941428738350962No Hit
AAACGACCTAGGGAGG50370.19883501706894213No Hit
AGCCTACCTTAACTCG48830.19275588412698913No Hit
GCTATGATGACCTGCG48730.19236113523465453No Hit
TTGCGATGACATTCAG47670.18817679697590767No Hit
TGTTCCTCTTTATGGC47340.18687412563120348No Hit
CAGCTCACTGTTTACG45630.18012391957228166No Hit
CAATGTGACCAACGCC43710.1725447408394572No Hit
CCTGTTGACCCTAGTA43330.1710446950485857No Hit
CTGCCTTGACCATAAC43020.16982097348234843No Hit
CACAGCTCTGGACTGA42720.16863672680534458No Hit
TAACCAGACATCACAC41410.16346551631576123No Hit
CATGATGTGTCATGTG41280.16295234275572623No Hit
ATGCCGCGATGCCACG41160.16247864408492468No Hit
CATGCAAACGAAGGCT40400.15947855250318166No Hit
CTATTCAACCGAACGA39800.15711005914917403No Hit
TGGGCCATGCGTTCTC39680.15663636047837248No Hit
ATGGGTACTTTCGGGC39490.15588633758293674No Hit
AGCTTGTGACTCACTC38730.15288624600119372No Hit
TTGTTTGTGATACGCT38630.15249149710885912No Hit
GGGCTGACTAATACAG38220.15087302665028723No Hit
ATTAGCGTGGTCGGTC37990.14996510419791761No Hit
CATTAGTCTATTCTGG37620.14850453329627958No Hit
AGAACGCGAGGAATAT37290.14720186195157536No Hit
CCGCTATTGTTCGCAA37200.14684658794847422No Hit
CACTATACTACGCCAC36720.1449517932652681No Hit
ATAGGAAACAGACTTC35100.13855686120944746No Hit
AACCAACACCGGAATG35100.13855686120944746No Hit
GGTAGCCTGTGCCACG34650.1367804911939417No Hit
AACGTCCCTTACAGAA34430.13591204363080558No Hit
GCAGATACTCGGCTCA34330.13551729473847096No Hit
TTGTAACCTCATCCTC34090.13456989739686792No Hit
CCCAATGCTGGAATGC33890.13378039961219867No Hit
GCATCTCTGAGCCTAT33840.13358302516603138No Hit
AGCTCGGACCTTAAGC33820.13350407538756445No Hit
CTGCCATTGCACAGTT33780.13334617583063063No Hit
CCTACGGGATGGAACG33410.13188560492899257No Hit
ATAGCCAACATGGCCC33400.13184613003975912No Hit
TCAGGCTCTTAAAGCA33310.13149085603665794No Hit
CTGATTACTGCTTTGA33060.13050398380582146No Hit
TTGACGTGACCTCAAA32830.12959606135345184No Hit
GATACCATGTCTGGAT32150.1269117688855765No Hit
TGGAGTTACAAGTCTC32060.12655649488247536No Hit
CGATCAAACCGATCGC32030.12643807021477496No Hit
GGGATCAGATGAGTAA31970.12620122087937422No Hit
CGCTGAGTGTTAGCTG31740.12529329842700462No Hit
CGCTTACTGGCCACGT31630.12485907464543655No Hit
TGTTTGTTGCGCTCGA31370.12383272752536656No Hit
TCCAAAGTGAAGGCCA31130.12288533018376349No Hit
ATCTTACTGCGCACTG31130.12288533018376349No Hit
TGGGTGCACTTCTGAG30340.11976681393432011No Hit
CGCTTCCCTGCGCTGT30150.11901679103888435No Hit
TTCTAACACTACGTTG30050.11862204214654974No Hit
CATGTTACTAACGTGG29960.1182667681434486No Hit
TACGCCTTGGGTTGAG29590.11680619724181054No Hit
CTCGGATCTTTCTGAG29400.11605617434637479No Hit
ATTGAGAGATGATCAC29090.1148324527801375No Hit
CGAGTCTCTTACTTTC28970.11435875410933598No Hit
AGCCGGTACGCGCAAA28840.11384558054930098No Hit
CCTCCCTTGTTTCGCC28570.11277975853999753No Hit
GTCCTGACTTTACGAC28310.11175341141992756No Hit
AGTGGTACTCAACGCC28310.11175341141992756No Hit
ACCTGCTTGCCAAACT28290.11167446164146062No Hit
CTTAGGCCTTATGACC28110.11096391363525834No Hit
CCTTCAGTGTAGCTTG28090.11088496385679142No Hit
AACAGCTACTCGAGAG27940.1102928405182895No Hit
TATCTCCCTGGAATTA27890.1100954660721222No Hit
AACCACTGAAAACATG27860.10997704140442181No Hit
TACAACCGATTCCATT27670.10922701850898606No Hit
GCGTCGATGCTTGAAC27580.10887174450588492No Hit
TTCACGCTGTGGGCAA27290.10772697271811454No Hit
AGAACAATGAGTCGGA27210.10741117360424687No Hit
GGGTCGTACCGCTCGA27120.10705589960114573No Hit
TGAGATAGACTCATCC27110.10701642471191226No Hit
GTGTACTGAATCACAC26890.10614797714877612No Hit
ATCGCAATGTAACTCG26840.10595060270260882No Hit
TGGTCCTTGTTTCTGT26730.10551637892104075No Hit
GCATGGGCTATGTGGG26700.10539795425334036No Hit
ACCTCCATGGAATTGA26540.104766356025605No Hit
TCCGGGATGCGAGTTT26470.10449003180097076No Hit
CATGTCAGAACAGGTG26350.10401633313016924No Hit
AGAGAATTGCCCGCAA26150.1032268353455No Hit
AGAACATGAAAGCTAA26040.10279261156393195No Hit
AGGTTACTGGTCCCGA26010.10267418689623156No Hit
CGAATTTCTGATAGCG25940.10239786267159734No Hit
TTCTATGACCAACGCC25890.10220048822543004No Hit
ATTCCTAACATTGCCA25890.10220048822543004No Hit
GCATATCGAGCCGCTA25870.10212153844696313No Hit
GTAAGAAACGACAAAT25790.10180573933309543No Hit
CCTTCTTTGGATCATC25670.1013320406622939No Hit
TCGACCTTGGGACCTC25580.10097676665919275No Hit
GGTCAGAGAACCAGTT25560.10089781688072583No Hit
GTTGCTAGACGCTCGA25380.10018726887452353No Hit
TCCCAGACTCTCATTT25370.10014779398529007No Hit
GCATAGTACGCTCCTT25370.10014779398529007No Hit
GAAAGTAACCCTGCGA25360.10010831909605662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)