FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4400616
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGCTGT428574397.38961545383647No Hit
CTAGCTGA247770.5630348114900278No Hit
CTAGCAGT88210.20044920983789544No Hit
CAAGCTGT77270.17558905389609092No Hit
CTAGCTAT71120.1616137377130838RNA PCR Primer, Index 38 (100% over 8bp)
CTAGATGT65680.14925183201624498No Hit
CTAGCTCT62790.1426845696147994No Hit
CTACCTGT58020.13184517803871096No Hit
CTAGCTGG57050.12964094117732608No Hit
CTAGCGGT55370.12582329382977292No Hit
ATAGCTGT50210.11409766269085964No Hit
CTCGCTGT48800.11089356580987753No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)