FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3550495
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT338988795.47646173280063No Hit
CTTTCCTA658621.8550089494563433No Hit
CTTTCCAT103190.2906355311020013No Hit
CTTTACTT102120.2876218668101208No Hit
CTTTCATT95070.267765480587918No Hit
CATTCCTT75450.21250558020783017TruSeq Adapter, Index 27 (100% over 8bp)
CTATCCTT72230.20343642224534886No Hit
ATTTCCTT70510.19859202730886819No Hit
CGTTCCTT57840.16290686228258314No Hit
CTTTCCGT54850.15448550131742195No Hit
CTTACCTT50970.1435574476235004No Hit
CTGTCCTT49560.13958617037905982No Hit
CTTTCGTT48310.1360655345240593No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)