FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004946716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3864334
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTAGTGAACGTAA204970.5304148140404013No Hit
GATGAATGACTCCTAC190550.4930991989822826No Hit
CGACCAGACGCGCTGT189730.49097722919395687No Hit
TAGCTTTACCCTATCC176010.4554730517600187No Hit
CCAGGACTGTAACTCG133510.34549291029191576No Hit
ATCTGTACTGGGACTA126850.3282583751818554No Hit
CTTCCTTTGGCTCAGC122320.3165357859853729No Hit
GTTCTCGACCGTAGAG122250.31635464222295484No Hit
CGATCAAACCCGGCTA115220.29816263294011336No Hit
AGACCATTGAATCCAC108430.28059168798556233No Hit
TGCTGCTACTTTCTTG101390.2623738010223754No Hit
GGGGGGGGGGGGGGGG97620.2526179155321461No Hit
GGCAACAGAATAGCAA95850.24803756611100386No Hit
ACAGCCGACTTGCGTA93180.24112822545877244No Hit
AACTTACCTCACCTAA85030.22003791597724212No Hit
CGCGTTGGAGCGGTTC81260.2102820304870128No Hit
ATCGAAAGATTTCCGC77090.19949103778296598No Hit
CCCTGAACTAGCTTTG74790.19353917130351567No Hit
GTAACTGTGTGAAAGT74500.19278871857349805No Hit
AGTGGAGTGAGTTGAA73720.19077025950655402No Hit
CAATTCGGATAACGGG71270.18443022782192223No Hit
GGGTAGAACCAGTCTA66390.17180191981334947No Hit
TTCCTAAACTAATGCC64850.16781675704015234No Hit
AAGAACCCTTAACCCA64020.16566890957148114No Hit
TTTAGTCTGAGCAATA62420.1615284807162114No Hit
GTGAAGCGATCCCAGG61740.15976879845272174No Hit
CGCGATCGATATCTGG60030.1553437151136522No Hit
TGGAACACTCCTAGTA58100.15034932280698304No Hit
AGTCCTGCTCGAACGA57650.14918482719143844No Hit
TGCAGATACTGCCACG57290.14825323069900273No Hit
CTTTAACTGTCCGTAA57270.14820147533831185No Hit
CACATCCTGAGCCTAT56350.14582072874653174No Hit
TGATAGAGAGACTGTT55020.14237899726058875No Hit
GCAACTTGAAGGTGCA54790.14178381061264372No Hit
CATAGGTACAAGGTGC54330.14059343731675367No Hit
TCTCAGAGATGCATGT53200.1376692594377194No Hit
TCCGGGAACGTTCTCC53140.1375139933556468No Hit
TGCAGTATGACACCGC52960.13704819510942895No Hit
AGGTGTGGATCATGTG51900.13430516099281273No Hit
TCAAAGCACTCTGCAA51580.13347707522175878No Hit
CTATTGGGACTTGTGA50640.1310445732692878No Hit
TACGTTTACTGCACCG50630.13101869558894236No Hit
CTTAGATCTTGTCACA50370.13034587589996102No Hit
ATGTACGTGTACTTTC50280.1301129767768521No Hit
ATTTCTGACGGGTAGT49620.12840504987405332No Hit
TGAACTGTGAACGTAA49470.1280168846688718No Hit
TGGCAATTGAGTGGTG49410.12786161858679918No Hit
AGAGTACCTTAATCGC49060.12695589977470892No Hit
TCTATGGGATGCTCAA48920.1265936122498728No Hit
CGAGTCTCTGCTACGC48790.12625720240538213No Hit
GGTCTAAACTCCTACC47980.12416111029740183No Hit
NNNNNNNNNNNNNNNN46700.12084876721318602No Hit
GGGTGTGCTCGCAAAG46550.12046060200800449No Hit
TTCTTAGACGTCCCGA46130.11937373943349616No Hit
GAGTGTTACACCTGCG45560.11789871165380633No Hit
TCCATTTGAAATCTGC45470.1176658125306974No Hit
GCATGATACTTGCGTA45010.11647543923480734No Hit
TACTGTTTGCTTAAGC44990.11642368387411647No Hit
GGTCGCATGGTACTAC44650.11554384274237164No Hit
CTACAACGAATCTGTG44540.11525918825857186No Hit
CTCAATTTGGAATCTC43960.11375828279853656No Hit
CACCAGGTGCGCCACA43810.11337011759335502No Hit
GTAATCTCTACACAAA43300.1120503558957378No Hit
GGGATGTGACTGGAAG42970.11119639244433842No Hit
TTGCGGTCTTTTGGGT42790.11073059419812055No Hit
CATTCTATGCCTAGCG42570.11016128523052096No Hit
TCGGACCGATAGGCGA42460.10987663074672117No Hit
CTAGCTCACGAATAGT42410.109747242344994No Hit
TGCTAGTGAAGGCGTA42130.10902266729532178No Hit
AGACTACTGGCCCTAG42010.10871213513117654No Hit
CTTTCGTCTCGAACGA41850.10829809224564958No Hit
CGAGTCTCTTGGAACG41790.10814282616357697No Hit
CATAGTCGAAGATTCC40940.1059432233342149No Hit
ACCTCAGCTAATCCGT40730.10539979204696075No Hit
CACATTACTGATGGAC40720.1053739143666153No Hit
TGCGCAGACATCAGGG40690.10529628132557899No Hit
TGTAAGCACGCGCTGT40690.10529628132557899No Hit
AGCCAATTGTTATGGC40460.10470109467763397No Hit
TGAGGGATGTACGTTG40460.10470109467763397No Hit
ACCAAGTTGAAGCTAA40380.10449407323487048No Hit
TCCGGGTGAACACCTA40120.10382125354588916No Hit
TAGTACCGAACGAGCA39820.10304492313552606No Hit
ATGTCCAGACCGCTAG39730.10281202401241714No Hit
AGGCGATGACCTGCGA39190.1014146292737636No Hit
CTAACAGTGCATGATC38800.10040539974029161No Hit
AAAGCCTTGAACCAAA38670.10006898989580093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)