FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337559
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTAGATC324605397.2582956585936Illumina Single End PCR Primer 1 (100% over 8bp)
TGTAGCTC206330.6182062998736502No Hit
TGTAGATA160210.48002147677389373No Hit
AGTAGATC60610.18159978595134948No Hit
TGTAGAAC60250.18052115333391858Illumina DpnII expression Adapter 1 (100% over 8bp)
TGAAGATC55750.16703824561603256No Hit
TGTACATC53000.15879869089954662No Hit
TCTAGATC33820.10133154200420127ABI Dynabead EcoP Oligo (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)