FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2774634
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC165440.5962588218842557No Hit
CTCCTAGTGAACGTAA143620.5176178191429933No Hit
TAGCTTTACCCTATCC126630.4563845177417995No Hit
CCAGGACTGTAACTCG95460.34404537679564223No Hit
ATCTGTACTGGGACTA92410.333052935990837No Hit
CTTCCTTTGGCTCAGC88140.31766351886410965No Hit
GTTCTCGACCGTAGAG84680.30519340568882236No Hit
CGATCAAACCCGGCTA80340.2895517030354274No Hit
TGCTGCTACTTTCTTG69170.24929414113717344No Hit
GGGGGGGGGGGGGGGG65560.2362834161190269No Hit
ACAGCCGACTTGCGTA64380.23203060295520056No Hit
CGACCAGACGCGCTGT63400.2284986055818533No Hit
AACTTACCTCACCTAA58950.21246045424369486No Hit
CGCGTTGGAGCGGTTC58660.21141527134750024No Hit
GGCAACAGAATAGCAA55690.20071115685888663No Hit
AGTGGAGTGAGTTGAA53970.19451214106076695No Hit
ATCGAAAGATTTCCGC53420.19252989763694958No Hit
CCCTGAACTAGCTTTG53370.19234969368932983No Hit
GTAACTGTGTGAAAGT52720.190007042370273No Hit
CAATTCGGATAACGGG50010.1802399884092821No Hit
GGGTAGAACCAGTCTA46750.16849069102447387No Hit
TTCCTAAACTAATGCC45550.16416579628159966No Hit
TTTAGTCTGAGCAATA44940.16196730812063861No Hit
AAGAACCCTTAACCCA44350.15984090153872546No Hit
GTGAAGCGATCCCAGG43760.15771449495681233No Hit
TCCGGGAACGTTCTCC42760.15411041600441716No Hit
CGCGATCGATATCTGG42740.15403833442536927No Hit
TGGAACACTCCTAGTA41780.1505784186310699No Hit
TGCAGATACTGCCACG41740.1504342554729741No Hit
TGATAGAGAGACTGTT39600.14272152651484846No Hit
CTTTAACTGTCCGTAA39530.14246924098818078No Hit
AGTCCTGCTCGAACGA38720.1395499370367407No Hit
CACATCCTGAGCCTAT38600.13911744756245328No Hit
CATAGGTACAAGGTGC38170.13756769361292334No Hit
AGGTGTGGATCATGTG38000.1369550001910162No Hit
TCTCAGAGATGCATGT37900.13659459229577667No Hit
GCAACTTGAAGGTGCA37440.1349367159776749No Hit
CTTAGATCTTGTCACA37270.13432402255576772No Hit
TGCAGTATGACACCGC36970.13324279887004917No Hit
CTATTGGGACTTGTGA36500.13154888176242344No Hit
ATTTCTGACGGGTAGT35880.12931435281193845No Hit
TCAAAGCACTCTGCAA35870.1292783120224145No Hit
TACGTTTACTGCACCG35850.1292062304433666No Hit
TGGCAATTGAGTGGTG35060.1263590080709744No Hit
TGAACTGTGAACGTAA34850.12560215149097143No Hit
TCTATGGGATGCTCAA34640.12484529491096845No Hit
TGAGGGATGTACGTTG34540.1244848870157289No Hit
AGAGTACCTTAATCGC34300.12361990806715407No Hit
GGTCGCATGGTACTAC33920.12225035806524391No Hit
TTCTTAGACGTCCCGA33850.12199807253857625No Hit
CGAGTCTCTGCTACGC33720.12152954227476488No Hit
ATGTACGTGTACTTTC33520.12080872648428587No Hit
GGTCTAAACTCCTACC33500.12073664490523796No Hit
TCCATTTGAAATCTGC32900.11857419753380086No Hit
GGGTGTGCTCGCAAAG32470.11702444358427093No Hit
CTACAACGAATCTGTG32300.11641175016236374No Hit
CATTCTATGCCTAGCG32100.11569093437188473No Hit
CTAGCTCACGAATAGT31700.11424930279092665No Hit
GGGATGTGACTGGAAG31480.11345640542139973No Hit
GCATGATACTTGCGTA31130.11219497778806142No Hit
TACTGTTTGCTTAAGC30980.11165436594520214No Hit
GAGTGTTACACCTGCG30910.11140208041853449No Hit
CTCAATTTGGAATCTC30420.10963608173186085No Hit
TGTAAGCACGCGCTGT30280.10913151067852554No Hit
TTGCGGTCTTTTGGGT30250.10902338830995367No Hit
TCGGACCGATAGGCGA30060.10833861330899859No Hit
CGAGTCTCTTGGAACG30060.10833861330899859No Hit
TCCGGGTGAACACCTA30020.10819445015090279No Hit
CTTTCGTCTCGAACGA29970.10801424620328302No Hit
GTAATCTCTACACAAA29780.10732947120232796No Hit
CACCAGGTGCGCCACA29590.10664469620137286No Hit
CACATTACTGATGGAC29540.10646449225375312No Hit
AGCCAATTGTTATGGC29150.105058901462319No Hit
AGACTACTGGCCCTAG28740.10358122909183698No Hit
CATAGTCGAAGATTCC28370.10224771987945078No Hit
TGCGCAGACATCAGGG27990.10087816987754061No Hit
TGCTAGTGAAGGCGTA27910.10058984356134901No Hit
ACCAAGTTGAAGCTAA27850.10037359882420528No Hit
AGGCGATGACCTGCGA27770.10008527250801368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)