FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3610083
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATTGGTG353123597.8158950916087No Hit
TAATGGTG89690.2484430413372767No Hit
TATTGGTA82880.22957920912067675No Hit
AATTGGTG65800.18226727751134808No Hit
TATTGGGG60720.1681955788828124No Hit
TATTGGTC52460.14531521851436657No Hit
TATTGGAG50440.13971977929593307No Hit
TATTGCTG47300.13102191833262558No Hit
TATAGGTG45000.1246508736779736RNA PCR Primer, Index 39 (100% over 8bp)
TATTCGTG43400.1202188426138679No Hit
TTTTGGTG42830.11863993154728021RNA PCR Primer, Index 28 (100% over 8bp)
TATTGGTT41920.11611921387957008No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)