FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004946741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18367595
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1896351.0324432785021664No Hit
TGCTGCTACCGTTCTC1852981.0088310418429849No Hit
AAACGACCTCTTCAAG1830660.9966792059602795No Hit
ATGGGTTACAATCGAA1760880.9586883857140797No Hit
TATGCTTTGGACTGTT1738730.9466291041369325No Hit
AAAGCGGACTCGGGCT1603800.873168207378266No Hit
GCCTCTTGATCTGTGA1586420.8637058907276648No Hit
GCAGGTCTGGCTGTTA1544120.8406762017564086No Hit
TCCAAAGTGCTCACGG1531090.8335821864539152No Hit
TAGTGCATGAACCCGC1459150.7944153820900342No Hit
TAACTCACTGGTGTCC1376080.7491889928975459No Hit
TGTGGCTCTTACGATC1360940.7409462153319474No Hit
GAAGTATGAACAGGTG1345240.7323985529950982No Hit
TTCCCATCTCTTTGTT1235590.6727010259100334No Hit
TCACCTGACTCAATGC1179300.6420546620284256No Hit
GGAACTACTACCAAGG1145040.6234022472729827No Hit
TGAGAATGAGCAGTTT1136380.6186874220604276No Hit
GAGCCTGACCGAGTTT1105510.6018806490452343No Hit
ATGTCCAGAGTCGGTC1089720.5932839873701483No Hit
CTAGAGACTAATCTGC1071110.5831520130969787No Hit
TCGTATACTGCTAATC1069130.582074027655771No Hit
TTCGTCCGACCTGTGG1061130.5777185309236185No Hit
TCACACAGATTGGGAG1058610.5763465494529905No Hit
TGCGGTGACCTTAGTG1049690.5714901705966404No Hit
GAAGTAATGAGTCTGT1048780.5709947328433581No Hit
ATTCCTAACTGCTACA1024980.5580371300652045No Hit
TCCGCGAGAGAACAGC1020130.5553966101713371No Hit
AGGTCGAGAAGGGAGG997220.5429235564046354No Hit
ACTGATTCTCGGCTCA992330.5402612590271073No Hit
TGGACCTCTAGGGAGG976570.5316809304647668No Hit
TGGCCTGGACCCGAGT960490.5229263820331403No Hit
CCATGATGATTCGGGC930530.5066150467712295No Hit
GCAATCATGGAACGTG870530.4739488212800859No Hit
TTCGATGGAAATCCTG842080.4584595860263687No Hit
ACGAGGATGGAGCTGA837060.455726511826943No Hit
AAGGTGCGAACAGATA800800.435985222888462No Hit
TCCCAGACTGTCTTGA798880.4349399036727454No Hit
TTTCGTAACAGTACGG795200.43293637517595523No Hit
TGGTCCTTGGAGAGGG778440.4238116095220959No Hit
TCTCTCTACTCTGGAT758660.41304264385184886No Hit
TCGCCGAGAAGGTAAT670960.36529551092562745No Hit
TCCTTCACTTGTCACA669250.36436452349912984No Hit
TTCGATGGACAGGTGA665940.36256243672620175No Hit
TGATATCCTCGATGTT660260.3594700340463735No Hit
GCGGTTCGAGTACGTT657600.35802183138293286No Hit
AGCTGCTCTTGTCACA655410.3568295141525061No Hit
AGCCCAGTGAAAGTAC644470.3508733723712876No Hit
AACTCCCTGCTTCACC638830.3478027471751201No Hit
CTTTCGGTGCTTTGTT627890.3418466053939016No Hit
AACCCTCACGCGGTTC625270.3404201802141217No Hit
CCCTTGCCTCCTACGG603260.3284371198297872No Hit
ATGGCCGTGATACGCT600470.326918140344449No Hit
TCGGCTTACCTCCTAC582530.3171509389225971No Hit
TGTTTGTTGCAAGTTC554790.30204825400385843No Hit
AAACACGCTGCAGTTT554410.30184136790908117No Hit
GGGGGGGGGGGGGGGG536870.2922919413238369No Hit
TGGCCTTTGCGAAAGC533370.2903864115035202No Hit
ACCCTTGTGGGCCATC532620.28997808368488087No Hit
CTAACTGGAACCTTGA525410.2860526922550285No Hit
TACAACGTGAGCAGGT522480.28445749157687766No Hit
AAATAGGACTCTATCT514950.28035788027773917No Hit
CAATGTGTGGTTGACT512750.27916011867639723No Hit
TCAAAGGCTCTTAGTG509050.27714570143777667No Hit
ACAGCCGACAAACATG486710.26498297681324096No Hit
CTTTCGGTGAGCCATT480360.2615258012820949No Hit
TGAACTGTGTGCTGGC473010.25752418865942983No Hit
AGTGTTCGATTCGCCT450850.24545946271136748No Hit
CAGCCGATGTAATAGG449800.2448878037652725No Hit
GGACATTTGGCCGCTA443100.2412400752520948No Hit
GAGGTTGGAGGAATAT416180.22658382874840174No Hit
CGACGTTCTTGCTACA386920.2106535994505541No Hit
TTGGGAGGAATCTGTG381230.20755575239981067No Hit
GTGCTAATGGGAGTCA379880.20682076232625993No Hit
TGTACTGGAAATGCTC376540.20500234244058627No Hit
GGAACATCTTTACGAC354230.19285595092879607No Hit
AGGGACCCTAATCCTG351650.1914513032326769No Hit
TCTTGAACTCGTACAT348300.18962743897608805No Hit
GGACCACTGGCTAGAG346180.18847323234206764No Hit
GGGTGAAACCGACTAT344170.18737891378811433No Hit
TAACGAGCTTACTTTC341420.18588171178643692No Hit
TACGTGTGAAACGCAG337770.18389451640239235No Hit
TACCACCTGAAACATG333810.1817385455199769No Hit
CGGATGAGAACAGGCA333790.1817276567781465No Hit
AGCTTGTGAACCGTGC324200.17650650507047874No Hit
CATGATGTGCCTAGTA313620.1707463606422071No Hit
ACTTATCTGTCAGGTT312700.17024547851800959No Hit
AACTAGCACTGCCACG297530.16198636783966547No Hit
TCGGACCGAATGTCCA297110.16175770426122743No Hit
ACGTGTTGATCTTTGT295730.16100638107493115No Hit
TGGTAGGGATGTGCTA282960.15405391941623278No Hit
AACTACGACATTGCCA279390.15211027899950974No Hit
AACGAACCTGACACCC276920.15076551938345764No Hit
CGTCGTACTTGCGCCA276570.15057496640142598No Hit
CTCGGATCTAGTACGG276240.1503953021612247No Hit
GTAGATAGAGCTGAGA274080.14921931804354355No Hit
GTCCGAGTGGTCTTGA270770.14741723127061546No Hit
TTTAGGGACTGGGCAA268480.1461704703310368No Hit
CCTTCAGTGCAACGCC267310.14553347893395951No Hit
TACCACCTGTACTTTC265520.1445589365401404No Hit
GGTAGTTTGTCCAGCG265470.14453171468556444No Hit
GGCTTGCGAATACGCT262300.14280584910544902No Hit
AGTTTGCGAGCAGGAT262140.14271873917080596No Hit
TGAGGAGTGGTCCCAG260400.1417714186315628No Hit
GCCATAATGACCGTTA253240.13787324905628634No Hit
CTTTAACTGTTCGCCT248700.13540150466078985No Hit
AGAACGCGACACAGTT239580.13043623838613602No Hit
CAGGCCATGTCGTAGT236400.12870492843510542No Hit
CTTACATTGGAAGGCT235930.12844904300209145No Hit
CGATCGGTGCGAGGAT234660.12775760789586224No Hit
GTCCGTTACGGATGGT233740.12725672577166472No Hit
GGGATGTGATGCATGT233610.12718594894976723No Hit
TGCAATGACTCCGTAA227430.12382132772417946No Hit
CAAAGACCTGCTTTGA226320.1232170025525933No Hit
CCGTTACTGTTGGTTG225280.12265078797741348No Hit
GAGAAGAGATGCTACA219940.11974349390870172No Hit
GAACCATACTTTGGGT216520.11788151905570653No Hit
GAAAGTCCTTATCTGG215610.11738608130242417No Hit
TCCTCATCTTTCTGGA214920.11701041970927605No Hit
AATTCTGCTTAGCGAG214290.11666742434161903No Hit
AACTACGACGCTGTTA212600.11574732565695182No Hit
GATAAGAACATTGGTC208120.11330824748694644No Hit
CCCACCTCTTGACCTA206500.11242625939868556No Hit
TGTCTTCCTGAAATCA203120.11058606202935115No Hit
ATGGCCGTGGGCCAAA202540.11027028851627009No Hit
TCGTAGAACCGCACTG202050.11000351434142576No Hit
TCCGTAAGATTACGCA198390.108010874586466No Hit
CCTGAGGACTGACTCA197220.1073738831893887No Hit
GAAGGACACCCAGCTG196860.10717788583644183No Hit
TATGGGTACCCTTTGC192440.10477147389192759No Hit
ACAAGACCTAAGCTAA191200.10409637189844397No Hit
AGGTGATCTGTTAGTC187980.1023432844637526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)