FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946742

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946742
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18367595
Sequences flagged as poor quality0
Sequence length34
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGCC204590.11138638455388417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC32600.019.37095822
CCCACGA33500.018.97237219
ATCTCCG33900.018.7878110
TCTCCGA33700.018.7330611
TCCGAGC35650.017.98324213
CCACGAG35450.017.6133120
CACGAGA35850.017.49698621
CGAGCCC37050.017.22852715
GCCCACG38700.016.53131918
CTCCGAG40550.015.87921512
CCGAGCC43800.014.89303914
GACTAAG45900.013.94352326
ACTAAGC46250.013.7472427
CTTATAC50050.013.4597211
CTAAGCC48700.013.22753628
GAGACTA51150.012.75781224
TATACAC72900.012.6923813
CGAGACT51900.012.22185723
ACATCTC62350.011.5396098
AGACTAA58700.011.236670525
Produced by FastQC (version 0.11.5)