FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004946753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17814426
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGTCTCTTTGTT1897121.0649346770982124No Hit
TGCTGCTACCGTTCTC1734510.9736547223020265No Hit
AAACGACCTCTTCAAG1733040.9728295483671492No Hit
ATGGGTTACAATCGAA1668620.9366678443638881No Hit
TATGCTTTGGACTGTT1641600.9215003615609058No Hit
GCCTCTTGATCTGTGA1585330.8899136014823043No Hit
AAAGCGGACTCGGGCT1507210.846061500943112No Hit
TCCAAAGTGCTCACGG1462570.8210031577778594No Hit
GCAGGTCTGGCTGTTA1386910.7785319605582577No Hit
TAGTGCATGAACCCGC1383640.7766963695602653No Hit
GAAGTATGAACAGGTG1318590.7401810195849139No Hit
TAACTCACTGGTGTCC1316200.7388394102622222No Hit
TGTGGCTCTTACGATC1285880.7218194961768625No Hit
TTCCCATCTCTTTGTT1160400.6513822000214882No Hit
GAGCCTGACCGAGTTT1117970.6275644244726156No Hit
TCACCTGACTCAATGC1112220.624336703298776No Hit
GGAACTACTACCAAGG1105220.6204073036088842No Hit
TGAGAATGAGCAGTTT1072170.6018549236444666No Hit
CTAGAGACTAATCTGC1040030.583813365639735No Hit
ATGTCCAGAGTCGGTC1036080.5815960615290103No Hit
GAAGTAATGAGTCTGT1033050.5798951928061No Hit
TCGTATACTGCTAATC1011940.568045246027012No Hit
TCACACAGATTGGGAG992250.5569924060421594No Hit
TGCGGTGACCTTAGTG991840.5567622554888942No Hit
TTCGTCCGACCTGTGG990080.5557742921382929No Hit
ATTCCTAACTGCTACA977040.5484543818588373No Hit
TCCGCGAGAGAACAGC956750.5370647361862796No Hit
AGGTCGAGAAGGGAGG942810.5292396173752666No Hit
ACTGATTCTCGGCTCA937200.5260904841952246No Hit
TGGACCTCTAGGGAGG923840.5185909442156599No Hit
TGGCCTGGACCCGAGT910680.5112036727986633No Hit
CCATGATGATTCGGGC877260.4924435959934943No Hit
GCAATCATGGAACGTG832640.46739647968449843No Hit
TTCGATGGAAATCCTG809100.45418246987020516No Hit
ACGAGGATGGAGCTGA789350.44309594931658197No Hit
AAGGTGCGAACAGATA770120.4323013270256364No Hit
TCCCAGACTGTCTTGA760700.42701347772866777No Hit
TTTCGTAACAGTACGG747370.41953077803348815No Hit
TGGTCCTTGGAGAGGG732220.4110264344189367No Hit
TCTCTCTACTCTGGAT718960.40358302872065593No Hit
GCGGTTCGAGTACGTT700360.39314205240180067No Hit
TCCTTCACTTGTCACA643770.36137566262308984No Hit
TCGCCGAGAAGGTAAT637020.35758659863640846No Hit
TTCGATGGACAGGTGA628270.3526748490240438No Hit
AGCCCAGTGAAAGTAC623650.3500814452287152No Hit
TGATATCCTCGATGTT622470.349419060709562No Hit
AGCTGCTCTTGTCACA601640.33772628991806974No Hit
AACTCCCTGCTTCACC596670.3349364161382466No Hit
CTTTCGGTGCTTTGTT593650.3332411608434647No Hit
AACCCTCACGCGGTTC585850.3288626869032996No Hit
CCCTTGCCTCCTACGG574470.3224746056931613No Hit
ATGGCCGTGATACGCT571430.32076812354212253No Hit
TCGGCTTACCTCCTAC550280.308895723050521No Hit
TGTTTGTTGCAAGTTC525480.2949744212920472No Hit
GGGGGGGGGGGGGGGG519070.29137621386173207No Hit
AAACACGCTGCAGTTT517760.29064085477690943No Hit
CTAACTGGAACCTTGA514160.28862002065067943No Hit
ACCCTTGTGGGCCATC510030.28630167483364327No Hit
TGGCCTTTGCGAAAGC503990.2829111642440795No Hit
AAATAGGACTCTATCT493770.2771742406968375No Hit
TACAACGTGAGCAGGT492280.2763378399057034No Hit
CAATGTGTGGTTGACT483560.2714429305777239No Hit
TCAAAGGCTCTTAGTG478210.2684397465290209No Hit
CTTTCGGTGAGCCATT459020.25766757795058903No Hit
TGAACTGTGTGCTGGC449330.25222816609415316No Hit
AGTGTTCGATTCGCCT425080.23861560288274236No Hit
CAGCCGATGTAATAGG417970.23462445548343797No Hit
GAGGTTGGAGGAATAT415650.23332214015764524No Hit
ACAGCCGACAAACATG394190.2212757233940628No Hit
GGCTTGCGAATACGCT388760.21822763192033243No Hit
GATAAGAACATTGGTC387470.21750349969176666No Hit
GTGCTAATGGGAGTCA375320.21068318451574025No Hit
CGACGTTCTTGCTACA371440.2085051744019145No Hit
GTAAAGCCTGAAATCA361800.20309382968612066No Hit
TTGGGAGGAATCTGTG361330.2028299985640851No Hit
TGTACTGGAAATGCTC359560.20183642178535532No Hit
AGGGACCCTAATCCTG335170.18814527058014668No Hit
TCTTGAACTCGTACAT332450.1866184181292173No Hit
GGAACATCTTTACGAC326630.1833514029584787No Hit
TAACGAGCTTACTTTC324770.18230730532659317No Hit
GGACATTTGGCCGCTA324030.18189191164509033No Hit
GGGTGAAACCGACTAT321250.18033137862539045No Hit
TACGTGTGAAACGCAG316070.17742362285487054No Hit
CGGATGAGAACAGGCA315940.17735064828920113No Hit
TACCACCTGAAACATG314780.17669949062630477No Hit
GGACCACTGGCTAGAG314540.17656476835122276No Hit
AGCTTGTGAACCGTGC302550.169834268025251No Hit
CATGATGTGCCTAGTA299130.16791447560533243No Hit
AACTAGCACTGCCACG295880.16609011146359698No Hit
ACTTATCTGTCAGGTT295520.16588802805097397No Hit
ACGTGTTGATCTTTGT282940.1588263354654256No Hit
TCGGACCGAATGTCCA282350.158495143205849No Hit
TGGTAGGGATGTGCTA272910.15319606705262354No Hit
AACTACGACATTGCCA272130.152758219658607No Hit
CGTCGTACTTGCGCCA264950.14872777826240374No Hit
GTAGATAGAGCTGAGA264030.1482113428745894No Hit
CTCGGATCTAGTACGG263530.14793067146816855No Hit
AACGAACCTGACACCC262180.1471728586708323No Hit
GTCCGAGTGGTCTTGA257890.14476469800374148No Hit
GGTAGTTTGTCCAGCG256810.14415844776587244No Hit
TTTAGGGACTGGGCAA254660.14295156071826284No Hit
CCTTCAGTGCAACGCC251450.14114965028904103No Hit
TACCACCTGTACTTTC249090.13982488125073464No Hit
AGTTTGCGAGCAGGAT248350.13940948756923183No Hit
TGAGGAGTGGTCCCAG247320.13883130447200487No Hit
CTTTAACTGTTCGCCT237840.13350977460626573No Hit
GCCATAATGACCGTTA236000.13247690383063704No Hit
CAGGCCATGTCGTAGT227360.1276269019276849No Hit
AGAACGCGACACAGTT226080.12690838312724753No Hit
GAGAAGAGATGCTACA223770.12561168122958327No Hit
CTTACATTGGAAGGCT222480.1248875490010175No Hit
GTCCGTTACGGATGGT222270.12476966701032073No Hit
GGGATGTGATGCATGT221600.12439356732571681No Hit
TGCAATGACTCCGTAA215170.1207841330391448No Hit
CCGTTACTGTTGGTTG213730.11997579938865276No Hit
GAAAGTCCTTATCTGG213080.11961092656030568No Hit
GAACCATACTTTGGGT212490.11927973430072908No Hit
CGATCGGTGCGAGGAT209240.11745537015899361No Hit
TCCTCATCTTTCTGGA204940.11504159606377437No Hit
GAAGGACACCCAGCTG204750.11493494092933447No Hit
AACTACGACGCTGTTA204360.11471601723232622No Hit
GCTGGACACATTGGTC199730.11211700000886922No Hit
AATTCTGCTTAGCGAG199120.11177458089303578No Hit
GGCTTAAACTGCATCA198900.11165108547421061No Hit
CAAAGACCTGCTTTGA198050.1111739440832952No Hit
TCGTAGAACCGCACTG193730.10874894313181913No Hit
TGTCTTCCTGAAATCA192350.1079742900500976No Hit
ATGGCCGTGGGCCAAA192270.10792938262507026No Hit
TCCGTAAGATTACGCA191220.1073399726715865No Hit
CCCACCTCTTGACCTA191110.1072782249621739No Hit
GCGTTTAGAAGTCTAC190740.1070705281214225No Hit
CCTGAGGACTGACTCA189490.1063688496053704No Hit
ACAAGACCTAAGCTAA188920.10604888420205064No Hit
ATAGCCAACATCTGTG185430.10408979778523315No Hit
TATGGGTACCCTTTGC183550.1030344732970908No Hit
GCGTTAATGGTACGTT180260.10118765544284167No Hit
AGGTGATCTGTTAGTC178870.10040738893299173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)