FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17814426
Sequences flagged as poor quality0
Sequence length34
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCCGG198450.11139848120843186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCCGG29800.020.91279228
ACATCCG31550.019.62003327
ACGAGAC32500.019.47394422
TCCGAGC34050.018.86928213
CGAGACA34100.018.68376223
TCTCCGA34350.018.62298411
ATCTCCG34400.018.51458510
CCCACGA34700.018.27629919
CACGAGA36750.017.41143221
CCACGAG36450.017.36106320
CGAGCCC38450.016.8559415
CCGAGCC40750.016.1450214
CTCCGAG40150.016.0373312
GCCCACG40150.016.00408418
GACATCC46150.013.83785526
CTTATAC50500.013.5333491
TATACAC71300.012.6041233
ACATCTC60000.011.8748358
TACACAT71050.011.6240195
AGACATC57050.011.36544225
Produced by FastQC (version 0.11.5)