FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004946757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3146169
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT554761.7632873504252313No Hit
GAAGCAGTGGAACCTT181680.5774642112359508No Hit
GATACGTACCGCTCGA160130.5089682086372347No Hit
GTTCTTCCTAACGTAA159880.5081735914377136No Hit
CGCTTAAGATAATAGG130240.4139637762624958No Hit
CATGGAGGATCGACAT127590.40554083394757245No Hit
TGTCCCATGAGACAAT123460.39241375781148435No Hit
AAAGCATGATCTTTGT115780.36800311744219716No Hit
TGAGCGCACAGGACAC109400.3477244865104195No Hit
TGTCGAAACTTCGCAA107130.34050936233876816No Hit
GGGGGGGGGGGGGGGG101970.32410846334065335No Hit
TAATTGCCTGTCTGGC101570.32283707582141963No Hit
GGAAGGACTAGGTAAT88380.28091307237468804No Hit
AATGATGGAAAGCTAA83070.2640354030568606No Hit
CATGTCAGAGGTTTGT83060.26400361836887976No Hit
TCGACCTTGTTTCGCC77770.24718951842701395No Hit
ATGGGTACTTGGCTGC65720.20888896941009846No Hit
GCTATGATGACCTGCG62220.1977643286168035No Hit
AAACGACCTAGGGAGG58990.1874978743989913No Hit
TTGCGATGACATTCAG58910.18724359689514453No Hit
AGCCTACCTTAACTCG58870.18711645814322117No Hit
CAGCTCACTGTTTACG57970.18425583622494532No Hit
TGTTCCTCTTTATGGC56220.17869351582829784No Hit
CCTGTTGACCCTAGTA53510.17007986538548947No Hit
CTGCCTTGACCATAAC53080.1687131238023132No Hit
CACAGCTCTGGACTGA51810.1646764684287462No Hit
CAATGTGACCAACGCC51760.164517544988842No Hit
CATGCAAACGAAGGCT51240.16286474121383815No Hit
TAACCAGACATCACAC51220.16280117183787648No Hit
GTGAGATCTCGATTAC51140.16254689433402972No Hit
CATGATGTGTCATGTG50120.15930485615998377No Hit
AGCTTGTGACTCACTC49790.15825596145661597No Hit
ATGGGTACTTTCGGGC48730.15488678453064664No Hit
CTATTCAACCGAACGA48670.1546960764027616No Hit
ATGCCGCGATGCCACG47750.15177188510852405No Hit
TGGGCCATGCGTTCTC47410.1506912057171754No Hit
CATTAGTCTATTCTGG47080.1496423110138076No Hit
AGAACGCGAGGAATAT46540.14792593786284208No Hit
TTGTTTGTGATACGCT46010.14624134939985742No Hit
ATTAGCGTGGTCGGTC45620.14500174656860457No Hit
CCGCTATTGTTCGCAA45370.14420712936908348No Hit
GGGCTGACTAATACAG44790.1423636174661946No Hit
CACTATACTACGCCAC44100.14017047399551646No Hit
GGGTCGTACCGCTCGA44020.1399161964916697No Hit
GCATCTCTGAGCCTAT43420.13800911521281914No Hit
GCAGATACTCGGCTCA42860.13622917268589194No Hit
ATAGGAAACAGACTTC41810.13289178044790345No Hit
AGCTCGGACCTTAAGC41460.13177931636857398No Hit
GGTAGCCTGTGCCACG41420.1316521776166506No Hit
CCCAATGCTGGAATGC41290.13123897667289963No Hit
TTGTAACCTCATCCTC40590.12901404851424064No Hit
CTGCCATTGCACAGTT40510.1287597710103939No Hit
GGCTTGCGAGTACGTT40510.1287597710103939No Hit
TTGACGTGACCTCAAA40420.12847370881856632No Hit
ATAGCCAACATGGCCC40180.1277108763070261No Hit
CTGATTACTGCTTTGA40170.12767909161904525No Hit
GATACCATGTCTGGAT40120.12752016817914105No Hit
AACCAACACCGGAATG39710.12621699597192648No Hit
AACGTCCCTTACAGAA39650.12602628784404143No Hit
TGGAGTTACAAGTCTC39380.12516810126855868No Hit
TGTTTGTTGCGCTCGA38880.12357886686951655No Hit
GGGATCAGATGAGTAA38130.12119501527095335No Hit
CGCTTACTGGCCACGT37790.1201143358796047No Hit
CCTACGGGATGGAACG37780.12008255119162385No Hit
ATCTTACTGCGCACTG37610.11954221149594953No Hit
CGATCAAACCGATCGC37470.11909722586421771No Hit
CGCTGAGTGTTAGCTG37130.11801654647286906No Hit
CATGTTACTAACGTGG36770.11687229770555874No Hit
CTCGGATCTTTCTGAG36060.1146155848589189No Hit
CGCTTCCCTGCGCTGT35940.11423416860314878No Hit
TGGGTGCACTTCTGAG35400.11251779545218327No Hit
TCAGGCTCTTAAAGCA35380.11245422607622159No Hit
TCCAAAGTGAAGGCCA35170.1117867476286239No Hit
TTCTAACACTACGTTG35030.11134176199689208No Hit
AGCCGGTACGCGCAAA34990.11121462324496871No Hit
CCTCCCTTGTTTCGCC34940.11105569980506451No Hit
TACAACCGATTCCATT34810.11064249886131355No Hit
AGTGGTACTCAACGCC34680.1102292979175626No Hit
CCTTCAGTGTAGCTTG34540.1097843122858308No Hit
ATTGAGAGATGATCAC34360.10921218790217564No Hit
AACAGCTACTCGAGAG34330.1091168338382331No Hit
CTTAGGCCTTATGACC34300.10902147977429058No Hit
GCATAGTACGCTCCTT34260.10889434102236721No Hit
ACCTGCTTGCCAAACT34070.10829043195073118No Hit
GTCCTGACTTTACGAC33880.10768652287909519No Hit
TATCTCCCTGGAATTA33850.10759116881515265No Hit
AACCACTGAAAACATG33820.10749581475121013No Hit
AGAACAATGAGTCGGA33400.10616085785601471No Hit
TACGCCTTGGGTTGAG33260.10571587222428293No Hit
CGAGTCTCTTACTTTC33220.10558873347235956No Hit
ATCGCAATGTAACTCG33010.10492125502476186No Hit
TGAGATAGACTCATCC32830.1043491306411067No Hit
GTGTACTGAATCACAC32720.10399949907331742No Hit
GCATGGGCTATGTGGG32690.1039041450093749No Hit
GTAAGAAACGACAAAT32490.10326845124975804No Hit
CATGTCAGAACAGGTG32430.10307774312187298No Hit
TGGTCCTTGTTTCTGT32250.10250561873821783No Hit
AGAACATGAAAGCTAA32240.10247383405023697No Hit
GTTGCTAGACGCTCGA31950.10155207809879253No Hit
GCATATCGAGCCGCTA31920.10145672403485001No Hit
TTCACGCTGTGGGCAA31910.10142493934686916No Hit
ACCTCCATGGAATTGA31740.10088459965119484No Hit
TTCTATGACCAACGCC31610.10047139870744388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)