FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2006733
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT356851.778263476008019No Hit
GAAGCAGTGGAACCTT115450.5753132080849819No Hit
GTTCTTCCTAACGTAA107960.5379888605011229No Hit
CGCTTAAGATAATAGG82820.41271060973233603No Hit
CATGGAGGATCGACAT81170.40448829017113885No Hit
TGTCCCATGAGACAAT79230.39482083565676146No Hit
AAAGCATGATCTTTGT76610.3817647888383756No Hit
GATACGTACCGCTCGA71480.35620084983901695No Hit
TGAGCGCACAGGACAC70330.35047014226606127No Hit
TGTCGAAACTTCGCAA68350.34060335879262466No Hit
TAATTGCCTGTCTGGC66960.33367667746531304No Hit
GGAAGGACTAGGTAAT61760.30776391278760057No Hit
GGGGGGGGGGGGGGGG57090.2844922568174241No Hit
AATGATGGAAAGCTAA54380.2709877198411548No Hit
CATGTCAGAGGTTTGT52980.2640112062740783No Hit
TCGACCTTGTTTCGCC49320.2457726065201499No Hit
GTGAGATCTCGATTAC44230.22040799647985057No Hit
GCTATGATGACCTGCG43150.21502611458524878No Hit
ATGGGTACTTGGCTGC39970.1991794623400323No Hit
CAGCTCACTGTTTACG38710.19290060012966348No Hit
AGCCTACCTTAACTCG38540.19205345205366137No Hit
AAACGACCTAGGGAGG38120.18996049798353842No Hit
TTGCGATGACATTCAG38060.18966150454494943No Hit
TGTTCCTCTTTATGGC36830.18353213905387514No Hit
CTGCCTTGACCATAAC34320.17102424687290238No Hit
CAATGTGACCAACGCC34170.1702767632764299No Hit
CACAGCTCTGGACTGA33020.16454605570347425No Hit
CCTGTTGACCCTAGTA32680.16285175955146997No Hit
AGCTTGTGACTCACTC32510.16200461147546785No Hit
TAACCAGACATCACAC32460.16175545027664368No Hit
CATGCAAACGAAGGCT32290.16090830220064153No Hit
CATGATGTGTCATGTG32050.15971232844628558No Hit
ATGGGTACTTTCGGGC31250.15572574926509902No Hit
ATGCCGCGATGCCACG31080.1548786011890969No Hit
CTATTCAACCGAACGA30560.15228732472132564No Hit
ATTAGCGTGGTCGGTC30540.15218766024179597No Hit
TTGTTTGTGATACGCT30100.1499950416921434No Hit
AGAACGCGAGGAATAT30090.14994520945237855No Hit
CATTAGTCTATTCTGG29090.1449619854758954No Hit
CCGCTATTGTTCGCAA28810.1435666827624801No Hit
CACTATACTACGCCAC28720.14311819260459663No Hit
GCAGATACTCGGCTCA28010.13958010358129358No Hit
GCATCTCTGAGCCTAT27870.13888245222458592No Hit
GGGTCGTACCGCTCGA27120.13514503424222354No Hit
AACGTCCCTTACAGAA26880.1339490604878676No Hit
AGCTCGGACCTTAAGC26690.13300224793233578No Hit
AACCAACACCGGAATG26680.13295241569257096No Hit
ATAGGAAACAGACTTC26350.13130795178033153No Hit
CTGCCATTGCACAGTT26260.13085946162244802No Hit
CCCAATGCTGGAATGC26040.12976315234762173No Hit
GGGATCAGATGAGTAA26000.1295638233885624No Hit
CTGATTACTGCTTTGA25850.12881633979208992No Hit
TTGACGTGACCTCAAA25760.12836784963420644No Hit
GATACCATGTCTGGAT25650.1278196949967933No Hit
TTGTAACCTCATCCTC25410.12662372124243734No Hit
TGGGCCATGCGTTCTC25320.12617523108455383No Hit
TGGAGTTACAAGTCTC25230.12572674092667038No Hit
CCTACGGGATGGAACG24780.12348429013725294No Hit
CGCTGAGTGTTAGCTG24680.12298596773960462No Hit
CGATCAAACCGATCGC24610.12263714206125079No Hit
TGTTTGTTGCGCTCGA24580.12248764534195632No Hit
CGCTTACTGGCCACGT24490.12203915518407282No Hit
TCCAAAGTGAAGGCCA24420.121690329505719No Hit
ATAGCCAACATGGCCC24340.12129167158760035No Hit
CTCGGATCTTTCTGAG24020.11969703991512573No Hit
CATGTTACTAACGTGG23990.11954754319583123No Hit
TCAGGCTCTTAAAGCA23920.1191987175174774No Hit
TGGGTGCACTTCTGAG23340.11630844761111718No Hit
CGAGTCTCTTACTTTC23100.11511247385676121No Hit
TACAACCGATTCCATT23050.11486331265793705No Hit
CGCTTCCCTGCGCTGT23030.11476364817840738No Hit
ATCTTACTGCGCACTG23020.11471381593864255No Hit
GCGTCGATGCTTGAAC22970.11446465473981841No Hit
GTAAGAAACGACAAAT22630.11277035858781412No Hit
AGTGGTACTCAACGCC22590.11257102962875479No Hit
AACAGCTACTCGAGAG22430.11177371379251749No Hit
TTCTAACACTACGTTG22180.11052790779839669No Hit
GGTAGCCTGTGCCACG22150.11037841107910219No Hit
CTTAGGCCTTATGACC22100.11012924988027803No Hit
CCTCCCTTGTTTCGCC22020.1097305919621594No Hit
GCATATCGAGCCGCTA21910.10918243732474624No Hit
ATTGAGAGATGATCAC21840.10883361164639244No Hit
GCGACAAGAAAGGCTG21830.1087837794066276No Hit
AGAACAATGAGTCGGA21750.10838512148850894No Hit
TGAGATAGACTCATCC21710.10818579252944961No Hit
GGGCTGACTAATACAG21590.10758780565227163No Hit
GTGTACTGAATCACAC21570.10748814117274197No Hit
CCTTCAGTGTAGCTTG21490.10708948325462331No Hit
GTTGCTAGACGCTCGA21310.10619250293885635No Hit
TACGCCTTGGGTTGAG21190.10559451606167836No Hit
ACCTCCATGGAATTGA21160.10544501934238386No Hit
CATGTCAGAACAGGTG21150.10539518710261904No Hit
ATCGCAATGTAACTCG21140.1053453548628542No Hit
TATCTCCCTGGAATTA20900.10414938110849825No Hit
AGCCGGTACGCGCAAA20830.10380055543014442No Hit
GCATAGTACGCTCCTT20800.10365105871084992No Hit
AACCACTGAAAACATG20770.10350156199155543No Hit
GAAAGTAACCCTGCGA20650.10290357511437745No Hit
TTCTATGACCAACGCC20640.10285374287461262No Hit
TGGTCCTTGTTTCTGT20470.10200659479861049No Hit
AGAACATGAAAGCTAA20450.10190693031908082No Hit
TTCACGCTGTGGGCAA20450.10190693031908082No Hit
GGTCAGAGAACCAGTT20260.100960117763549No Hit
CGAATTTCTGATAGCG20070.10001330520801721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)