FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3248685
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGAAGGG317378497.69442097340925No Hit
GAGAAGGA196620.6052295005517617No Hit
GAGCAGGG97360.29969049015216925No Hit
GAGAAGGC43900.1351315994009884TruSeq Adapter, Index 20 (100% over 8bp)
GAGAACGG42930.1321457759062513No Hit
GAGAAGTG38950.11989466507217536No Hit
GAGAAGCG37640.11586226427000464No Hit
GACAAGGG33560.10330333658080115No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)