FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15566685
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG189830.12194632318955512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG17000.09.7998325
TAGACTG19150.08.9920135
ATACCGT4550.08.6152116
CGTATAC4001.6370905E-118.3993173
ACCGGTC7750.08.310158
TATACAG19550.08.2351535
TCTATAC12100.08.2142083
TACACTG26450.07.99230155
GTCCTAG22250.07.8070061
TAGACAG25700.07.789755
GTCTTAG19550.07.7387331
CTTACAC21200.07.7257863
GTCTTAC14600.07.6759121
CCTAGAC22350.07.64143663
GTGTAGG23650.07.6410211
TAGGACA17950.07.5650144
CCTATAC13150.07.55832053
CTAGGAC23350.07.55398853
CTATACT18350.07.55268864
CCTACAC23950.07.5400963
Produced by FastQC (version 0.11.5)