FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004946784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3783240
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT636791.6831868980027702No Hit
GAAGCAGTGGAACCTT247860.655152726234656No Hit
GTTCTTCCTAACGTAA197080.5209291506750827No Hit
GATACGTACCGCTCGA154860.4093316839534367No Hit
CGCTTAAGATAATAGG153750.40639769086814476No Hit
CATGGAGGATCGACAT151160.3995517070024635No Hit
TGTCCCATGAGACAAT147020.38860870576542855No Hit
TGAGCGCACAGGACAC139330.3682822131294869No Hit
AAAGCATGATCTTTGT138570.3662733529990167No Hit
TAATTGCCTGTCTGGC127810.337832122730781No Hit
TGTCGAAACTTCGCAA125430.3315412186379928No Hit
GGAAGGACTAGGTAAT114220.3019105317135577No Hit
CATGTCAGAGGTTTGT100800.2664382909886764No Hit
AATGATGGAAAGCTAA99130.26202408517566955No Hit
GGGGGGGGGGGGGGGG95410.2521912434844208No Hit
TCGACCTTGTTTCGCC95290.2518740550427676No Hit
GTGAGATCTCGATTAC81420.2152123576616868No Hit
ATGGGTACTTGGCTGC78070.20635751366553534No Hit
AAACGACCTAGGGAGG75350.19916790898806314No Hit
CAGCTCACTGTTTACG71160.1880927459003394No Hit
AGCCTACCTTAACTCG70940.18751123375730855No Hit
GCTATGATGACCTGCG69830.18457724067201658No Hit
TGTTCCTCTTTATGGC69390.1834142163859549No Hit
TTGCGATGACATTCAG69080.18259481291168417No Hit
CCTGTTGACCCTAGTA63880.1688499804400461No Hit
CAATGTGACCAACGCC63400.16758122667343334No Hit
CTGCCTTGACCATAAC62900.1662596081665451No Hit
TAACCAGACATCACAC62090.16411858618538608No Hit
AGCTTGTGACTCACTC61890.16358993878263076No Hit
CACAGCTCTGGACTGA60510.1599422717036191No Hit
CATGATGTGTCATGTG60310.1594136243008638No Hit
ATGCCGCGATGCCACG60000.15859422082659308No Hit
CATGCAAACGAAGGCT59700.1578012497224601No Hit
ATGGGTACTTTCGGGC58300.15410071790317295No Hit
CTATTCAACCGAACGA57950.15317558494835115No Hit
AGAACGCGAGGAATAT56440.14918429705754857No Hit
TGGGCCATGCGTTCTC56020.1480741375117624No Hit
ATTAGCGTGGTCGGTC55370.14635603345280765No Hit
TTGTTTGTGATACGCT55370.14635603345280765No Hit
CATTAGTCTATTCTGG54360.14368636406889335No Hit
GGGCTGACTAATACAG53060.14025015595098383No Hit
CACTATACTACGCCAC52980.1400386969898817No Hit
CCGCTATTGTTCGCAA52630.1391135640350599No Hit
AACCAACACCGGAATG51210.1353601674754972No Hit
TTGACGTGACCTCAAA50630.1338270900075068No Hit
CCCAATGCTGGAATGC50100.1324261743902052No Hit
GGCTTGCGAGTACGTT50040.13226758016937865No Hit
GGGTCGTACCGCTCGA50020.1322147154291031No Hit
CTGCCATTGCACAGTT50020.1322147154291031No Hit
AGCTCGGACCTTAAGC49910.1319239593575877No Hit
TTGTAACCTCATCCTC49740.13147460906524566No Hit
ATAGGAAACAGACTTC49580.1310516911430414No Hit
GGTAGCCTGTGCCACG49550.13097239403262811No Hit
AACGTCCCTTACAGAA49120.1298358021167042No Hit
CTGATTACTGCTTTGA47920.12666391770017232No Hit
CCTACGGGATGGAACG47800.12634672925851914No Hit
ATAGCCAACATGGCCC47550.12568592000507503No Hit
TGGAGTTACAAGTCTC47420.12534229919328407No Hit
TCAGGCTCTTAAAGCA47290.12499867838149312No Hit
GCAGATACTCGGCTCA45980.12153603789344582No Hit
CGCTTACTGGCCACGT45970.12150960552330806No Hit
TGTTTGTTGCGCTCGA45840.12116598471151711No Hit
CGATCAAACCGATCGC45730.12087522864000169No Hit
CGCTGAGTGTTAGCTG45520.12032014886710861No Hit
TCCAAAGTGAAGGCCA44620.11794123555470971No Hit
TGGGTGCACTTCTGAG44580.11783550607415866No Hit
CGCTTCCCTGCGCTGT43730.1155887546124486No Hit
GCGTCGATGCTTGAAC43720.11556232224231083No Hit
TTCTAACACTACGTTG43680.11545659276175975No Hit
ATCTTACTGCGCACTG43510.11500724246941774No Hit
CTCGGATCTTTCTGAG43370.11463718928748903No Hit
AGCCGGTACGCGCAAA42910.11342130026115183No Hit
GTAAGAAACGACAAAT42820.11318340892991192No Hit
CGAGTCTCTTACTTTC42700.11286622048825873No Hit
GATACCATGTCTGGAT42650.11273405863756993No Hit
GCGACGTTGCTTAGTG42370.11199395227371248No Hit
CATGTTACTAACGTGG42330.11188822279316142No Hit
CCTCCCTTGTTTCGCC41810.11051373954599761No Hit
AGTGGTACTCAACGCC41340.10927141814952263No Hit
CCTTCAGTGTAGCTTG41330.10924498577938486No Hit
TACGCCTTGGGTTGAG41290.10913925629883381No Hit
AGAACAATGAGTCGGA40470.10697180194753704No Hit
ACCTGCTTGCCAAACT40460.10694536957739927No Hit
AACAGCTACTCGAGAG40330.10660174876560831No Hit
AACCACTGAAAACATG40320.10657531639547055No Hit
ATCGCAATGTAACTCG40070.10591450714202641No Hit
GTCCTGACTTTACGAC39970.10565018344064875No Hit
TACAACCGATTCCATT39920.10551802158995993No Hit
CTTAGGCCTTATGACC39800.10520083314830675No Hit
TGAGATAGACTCATCC39740.10504223892748014No Hit
TTCACGCTGTGGGCAA39580.1046193210052759No Hit
ATTGAGAGATGATCAC39500.10440786204417378No Hit
GAAAGTAACCCTGCGA39350.10401137649210729No Hit
GCGACAAGAAAGGCTG38750.10242543428384138No Hit
TGGTCCTTGTTTCTGT38600.10202894873177488No Hit
GGTCAGAGAACCAGTT38460.10165889554984617No Hit
TTCTATGACCAACGCC38270.10115668051722862No Hit
GTGTACTGAATCACAC38260.10113024814709085No Hit
TCCGGGATGCGAGTTT38060.10060160074433554No Hit
ACCTCCATGGAATTGA37980.10039014178323342No Hit
CCTTCTTTGGATCATC37880.10012581808185578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)