FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3783240
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGATC370244897.86447595182965Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGATA184330.4872278787494317No Hit
ATATGATC101050.26709910024212047No Hit
ATCAGATC99170.2621298146562206Illumina PCR Primer Index 9 (100% over 8bp)
ATCTGCTC66520.17582812615641621No Hit
ATCTGAAC56620.14966007972002832No Hit
AACTGATC56390.14905213520685973No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)