FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31350270
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGG928590.29619840594674307No Hit
CTTAGAGACAATCGCT329920.10523673320835833No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGGG400.00295159610.00243510
GTCGCAG553.892723E-510.0019249
CCAGGGT19300.010.0019239
GAGCCGT19400.010.0012858
TGAGGTC20800.010.0012858
ACGGTAT11500.010.0012858
GATCCAG15000.010.0012848
GAAGGTA14200.010.0012848
ATCTCCG751.2559212E-710.0010776
AGGAGGC2300.010.0010776
GCACGGC3650.010.0010776
CAGATCG5600.010.0010776
CGTGTCA5200.010.0010776
GTCTTAA8450.010.0010776
CGCCGCA456.959106E-410.0010777
CATGGTC3850.010.0010766
GGATCGG4550.010.0010767
CTGCAGC2050.010.0010767
AGGATCG9350.010.0010766
ACGGAGC4600.010.0010455
Produced by FastQC (version 0.11.5)