FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3311166
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA322254897.32366181580748No Hit
GGCAAGGC122840.37098713866958044No Hit
GGCAAGAA103490.3125485101018795No Hit
GGCAACGA81130.2450194282014251No Hit
GGCAAGCA68140.20578853491489102Clontech Universal Primer Mix Long (100% over 8bp)
GCCAAGGA53570.16178590864970227RNA PCR Primer, Index 1 (100% over 8bp)
CGCAAGGA52640.15897723037745617No Hit
GGAAAGGA51930.15683297062122528No Hit
GGCAGGGA47840.14448082639166987No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)