FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4644751
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAACAA453432997.6226497394586No Hit
GCCAAAAA143500.30895089962841926No Hit
CCCAACAA136560.29400930211328874RNA PCR Primer, Index 36 (100% over 8bp)
GCCCACAA91830.19770704608277173No Hit
GACAACAA78220.16840515239675927No Hit
GCAAACAA78000.16793149944959376No Hit
GCCACCAA71480.15389414846996102No Hit
GCCAACCA70180.15109529014580114No Hit
ACCAACAA54360.11703533730871686No Hit
TCCAACAA54140.11656168436155136No Hit
GCCAACAC47120.10144784941108792No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)