FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004946819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3615655
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAATGACTCCTAC195800.5415339682574803No Hit
CTCCTAGTGAACGTAA189030.522809836668598No Hit
CGACCAGACGCGCTGT182140.5037538150072394No Hit
TAGCTTTACCCTATCC163700.452753373870018No Hit
CCAGGACTGTAACTCG129870.35918803093768625No Hit
GTTCTCGACCGTAGAG117390.3246714634001308No Hit
ATCTGTACTGGGACTA115740.320107974903579No Hit
CTTCCTTTGGCTCAGC113530.3139956660688036No Hit
CGATCAAACCCGGCTA105250.29109525106792544No Hit
AGACCATTGAATCCAC104470.2889379655968282No Hit
NNNNNNNNNNNNNNNN103710.28683599513781044No Hit
GGGGGGGGGGGGGGGG100620.2782898257715407No Hit
TGCTGCTACTTTCTTG93680.2590955165799834No Hit
ACAGCCGACTTGCGTA90350.2498855670687607No Hit
GGCAACAGAATAGCAA88160.2438285732460647No Hit
AACTTACCTCACCTAA80580.22286418366796612No Hit
CGCGTTGGAGCGGTTC75600.2090907456601916No Hit
ATCGAAAGATTTCCGC74520.20610373500790313No Hit
GTAACTGTGTGAAAGT72770.20126367145095425No Hit
CCCTGAACTAGCTTTG69900.1933259672175581No Hit
AGTGGAGTGAGTTGAA67260.1860243856230752No Hit
CAATTCGGATAACGGG66420.18370115511573976No Hit
GGGTAGAACCAGTCTA61670.1705638397468785No Hit
TTCCTAAACTAATGCC59760.1652812560932943No Hit
AAGAACCCTTAACCCA59730.1651982835751752No Hit
CGCGATCGATATCTGG59060.16334523067051473No Hit
GTGAAGCGATCCCAGG58010.16044119253634542No Hit
TGGAACACTCCTAGTA56280.15565644399147596No Hit
TTTAGTCTGAGCAATA56210.155462841449198No Hit
TGCAGATACTGCCACG55270.15286303588146546No Hit
TGATAGAGAGACTGTT54060.1495164776506608No Hit
CTTTAACTGTCCGTAA53180.1470826171191665No Hit
CACATCCTGAGCCTAT53090.14683369956480916No Hit
AGTCCTGCTCGAACGA52240.14448281155143397No Hit
TCCGGGAACGTTCTCC51530.1425191286226147No Hit
GCAACTTGAAGGTGCA50900.1407767057421131No Hit
CATAGGTACAAGGTGC50660.14011292559716013No Hit
TGCAGTATGACACCGC50120.1386194202710159No Hit
TCTCAGAGATGCATGT49800.13773438007774524No Hit
AGGTGTGGATCATGTG48930.13532817705229067No Hit
GAGTGTTACACCTGCG48020.13281134400267725No Hit
TCAAAGCACTCTGCAA47740.13203693383356543No Hit
TACGTTTACTGCACCG47600.13164972874900951No Hit
CTTAGATCTTGTCACA47320.13087531857989768No Hit
CTATTGGGACTTGTGA47010.13001793589266675No Hit
TGGCAATTGAGTGGTG46710.12918821071147552No Hit
ATGTACGTGTACTTTC46490.12857974557860194No Hit
TTCTTAGACGTCCCGA46230.12786065042156952No Hit
CGAGTCTCTGCTACGC46110.12752876034909302No Hit
ATTTCTGACGGGTAGT46090.1274734453370136No Hit
TGAACTGTGAACGTAA45650.12625651507126648No Hit
AGAGTACCTTAATCGC45150.12487363976928108No Hit
GGTCGCATGGTACTAC44350.12266103928610446No Hit
GGTCTAAACTCCTACC44350.12266103928610446No Hit
TCTATGGGATGCTCAA44280.1224674367438265No Hit
GCATGATACTTGCGTA44270.12243977923778679No Hit
TCCATTTGAAATCTGC43150.1193421385613395No Hit
GGGTGTGCTCGCAAAG42920.11870601592242622No Hit
CACCAGGTGCGCCACA42910.11867835841638652No Hit
GGGATGTGACTGGAAG42220.11676999049964668No Hit
CTACAACGAATCTGTG41880.11582963529429662No Hit
TACTGTTTGCTTAAGC41830.11569134776409806No Hit
GTAATCTCTACACAAA41620.11511054013726421No Hit
AGACTACTGGCCCTAG41450.11464036253458916No Hit
CTCAATTTGGAATCTC41450.11464036253458916No Hit
CATTCTATGCCTAGCG40890.11309154219636554No Hit
TTGCGGTCTTTTGGGT40800.11284262464200814No Hit
CTAGCTCACGAATAGT40410.11176398190645956No Hit
TGTAAGCACGCGCTGT40140.11101722924338744No Hit
TCGGACCGATAGGCGA39710.10982795648367999No Hit
AGCCAATTGTTATGGC39680.10974498396556089No Hit
TGAGGGATGTACGTTG39200.10841742367565489No Hit
CTTTCGTCTCGAACGA39030.10794724607297987No Hit
CGAGTCTCTTGGAACG38990.10783661604882103No Hit
TCCGGGTGAACACCTA38820.107366438446146No Hit
ATGTCCAGACCGCTAG38510.10650905575891505No Hit
CACATTACTGATGGAC38260.10581761810792235No Hit
ACCTCAGCTAATCCGT38080.10531978299920762No Hit
TGCGCAGACATCAGGG37940.10493257791465171No Hit
AGGCGATGACCTGCGA37770.10446240031197666No Hit
ACCAAGTTGAAGCTAA37740.10437942779385753No Hit
TGCTAGTGAAGGCGTA37380.10338375757642805No Hit
TAGTACCGAACGAGCA37040.10244340237107799No Hit
ATAGCCAACCGTTCTC36820.10183493723820443No Hit
GCCATTCACCGGAAGT36770.10169664970800588No Hit
TCATCATTGGACTCGC36410.1007009794905764No Hit
CATAGTCGAAGATTCC36280.10034143191206019No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)