FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499804
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT447741.791100422273106No Hit
GAAGCAGTGGAACCTT161400.6456506190085303No Hit
GTTCTTCCTAACGTAA132290.5292014893967687No Hit
CGCTTAAGATAATAGG105060.42027294939923293No Hit
CATGGAGGATCGACAT105020.4201129368542494No Hit
GATACGTACCGCTCGA104570.4183127957231847No Hit
TGTCCCATGAGACAAT100360.4014714753636685No Hit
AAAGCATGATCTTTGT96180.38475016441288995No Hit
TGAGCGCACAGGACAC94370.3775095967523854No Hit
TAATTGCCTGTCTGGC85360.341466770994846No Hit
TGTCGAAACTTCGCAA83320.33330613120068614No Hit
GGAAGGACTAGGTAAT74180.2967432646719503No Hit
GGGGGGGGGGGGGGGG68330.2733414299681095No Hit
AATGATGGAAAGCTAA68230.2729413986056507No Hit
CATGTCAGAGGTTTGT66590.2663808842613261No Hit
TCGACCTTGTTTCGCC64340.2573801786060027No Hit
GTGAGATCTCGATTAC56400.22561768842677266No Hit
ATGGGTACTTGGCTGC52070.20829633043230592No Hit
AAACGACCTAGGGAGG50360.20145579413426012No Hit
GCTATGATGACCTGCG50100.20041571259186722No Hit
TTGCGATGACATTCAG47330.189334843851758No Hit
AGCCTACCTTAACTCG46590.18637461176956274No Hit
TGTTCCTCTTTATGGC46260.18505450827344866No Hit
CAGCTCACTGTTTACG45980.18393442045856395No Hit
CCTGTTGACCCTAGTA44020.17609380575437114No Hit
CTGCCTTGACCATAAC43890.17557376498317467No Hit
CAATGTGACCAACGCC42900.1716134544948324No Hit
TAACCAGACATCACAC41880.16753313459775246No Hit
CATGATGTGTCATGTG41470.1658930060116713No Hit
CATGCAAACGAAGGCT41360.16545297151296662No Hit
CACAGCTCTGGACTGA40510.16205270493206667No Hit
ATGCCGCGATGCCACG39250.15701230976508557No Hit
ATGGGTACTTTCGGGC38930.15573220940521737No Hit
AGAACGCGAGGAATAT38410.15365204632043153No Hit
CTATTCAACCGAACGA38210.15285198359551389No Hit
TGGGCCATGCGTTCTC38000.15201191773435038No Hit
AGCTTGTGACTCACTC37790.15117185187318688No Hit
TTGTTTGTGATACGCT37670.15069181423823627No Hit
ATTAGCGTGGTCGGTC37180.1487316605621881No Hit
CATTAGTCTATTCTGG36200.14481135321009167No Hit
CACTATACTACGCCAC36060.14425130930264932No Hit
CCGCTATTGTTCGCAA35670.14269118698905994No Hit
GGGCTGACTAATACAG35500.14201113367287996No Hit
GGTAGCCTGTGCCACG35040.1401709894055694No Hit
GCAGATACTCGGCTCA34210.13685072909716123No Hit
CTGCCATTGCACAGTT33940.1357706444185224No Hit
ATAGGAAACAGACTTC33760.13505058796609654No Hit
AACCAACACCGGAATG33580.1343305315136707No Hit
CCCAATGCTGGAATGC33570.1342905283774248No Hit
AGCTCGGACCTTAAGC33510.1340505095599495No Hit
AACGTCCCTTACAGAA33240.1329704248813107No Hit
GCATCTCTGAGCCTAT33180.1327304060638354No Hit
TTGACGTGACCTCAAA33160.13265039979134366No Hit
CCTACGGGATGGAACG33060.13225036842888482No Hit
TTGTAACCTCATCCTC32720.13089026179652485No Hit
CTGATTACTGCTTTGA32390.12957015830041074No Hit
ATAGCCAACATGGCCC32270.12909012066546016No Hit
TCAGGCTCTTAAAGCA32160.12865008616675547No Hit
TGGAGTTACAAGTCTC32140.12857007989426372No Hit
GATACCATGTCTGGAT32000.12801003598682137No Hit
GGGATCAGATGAGTAA31490.12596987603828141No Hit
CGCTGAGTGTTAGCTG31260.12504980390462614No Hit
CGATCAAACCGATCGC31240.12496979763213435No Hit
CGCTTACTGGCCACGT30620.1224896031848897No Hit
TGTTTGTTGCGCTCGA30410.12164953732372617No Hit
ATCTTACTGCGCACTG30270.12108949341628383No Hit
CTCGGATCTTTCTGAG29980.11992940246515327No Hit
TTCTAACACTACGTTG29960.11984939619266151No Hit
TCCAAAGTGAAGGCCA29690.1187693115140227No Hit
CATGTTACTAACGTGG29630.11852929269654741No Hit
TGGGTGCACTTCTGAG29580.11832927701531801No Hit
CGCTTCCCTGCGCTGT29480.11792924565285919No Hit
TACAACCGATTCCATT28910.11564906688684391No Hit
AGCCGGTACGCGCAAA28200.11280884421338633No Hit
TACGCCTTGGGTTGAG28030.11212879089720633No Hit
CTTAGGCCTTATGACC27960.11184876894348517No Hit
AGAACAATGAGTCGGA27940.1117687626709934No Hit
GCATGGGCTATGTGGG27930.11172875953474753No Hit
GTGTACTGAATCACAC27930.11172875953474753No Hit
ACCTGCTTGCCAAACT27910.11164875326225576No Hit
CCTTCAGTGTAGCTTG27770.11108870935481341No Hit
GCGTCGATGCTTGAAC27760.11104870621856752No Hit
ATTGAGAGATGATCAC27700.11080868740109226No Hit
AACAGCTACTCGAGAG27650.11060867171986284No Hit
CCTCCCTTGTTTCGCC27430.10972860272245342No Hit
CGAGTCTCTTACTTTC27390.10956859017746991No Hit
TATCTCCCTGGAATTA27310.10924856508750286No Hit
ATCGCAATGTAACTCG26730.10692838318524173No Hit
GTTGCTAGACGCTCGA26710.10684837691274995No Hit
TTCACGCTGTGGGCAA26660.10664836123152055No Hit
AGTGGTACTCAACGCC26640.10656835495902878No Hit
AACCACTGAAAACATG26640.10656835495902878No Hit
CATGTCAGAACAGGTG26610.10644834555029115No Hit
TCCGGGATGCGAGTTT26330.10532825773540645No Hit
TTCTATGACCAACGCC26290.10516824519042292No Hit
GGGTCGTACCGCTCGA26290.10516824519042292No Hit
ACCTCCATGGAATTGA26260.10504823578168529No Hit
TGGTCCTTGTTTCTGT26220.10488822323670177No Hit
GTCCTGACTTTACGAC26190.10476821382796411No Hit
TGAGATAGACTCATCC26090.10436818246550529No Hit
GTAAGAAACGACAAAT25920.1036881291493253No Hit
CCTTCTTTGGATCATC25720.10288806642440766No Hit
GCATAGTACGCTCCTT25630.10252803819819473No Hit
GAAAGTAACCCTGCGA25460.10184798488201476No Hit
CGGTTCACTGGAATGC25260.10104792215709711No Hit
AGAACATGAAAGCTAA25190.10076790020337595No Hit
GGTCAGAGAACCAGTT25020.10008784688719595No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)