FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2499804
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCCT244348297.74694336035945No Hit
TCACTCCA146080.5843658142798396No Hit
ACACTCCT75460.3018636661114231No Hit
GCACTCCT39780.15913247598611732No Hit
TCCCTCCT39350.1574123411275444No Hit
TCACACCT36550.14621146297869753No Hit
TCACTCAT33810.13525060364732597RNA PCR Primer, Index 45 (100% over 8bp)
TCACTACT26280.10512824205417703No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)