FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971413
Sequences flagged as poor quality0
Sequence length16
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT531431.788475718454486No Hit
GAAGCAGTGGAACCTT173640.5843684469308036No Hit
GTTCTTCCTAACGTAA160980.5417624544282468No Hit
CGCTTAAGATAATAGG121980.41051176662416167No Hit
CATGGAGGATCGACAT121440.4086944494084127No Hit
TGTCCCATGAGACAAT119680.402771341445972No Hit
AAAGCATGATCTTTGT112500.3786077532810148No Hit
TGTCGAAACTTCGCAA103210.34734316636563145No Hit
TGAGCGCACAGGACAC103120.34704028016300664No Hit
TAATTGCCTGTCTGGC101670.3421604468984958No Hit
GATACGTACCGCTCGA99630.3352950263056667No Hit
GGAAGGACTAGGTAAT91320.3073285335966424No Hit
GGGGGGGGGGGGGGGG85230.2868332338856968No Hit
AATGATGGAAAGCTAA78940.26566485372447385No Hit
CATGTCAGAGGTTTGT78790.26516004338676585No Hit
TCGACCTTGTTTCGCC74090.2493426528052479No Hit
GTGAGATCTCGATTAC67470.22706368990106726No Hit
GCTATGATGACCTGCG63260.21289534642272884No Hit
ATGGGTACTTGGCTGC60220.20266452357851297No Hit
AAACGACCTAGGGAGG57280.1927702409594358No Hit
TTGCGATGACATTCAG55900.18812598585252202No Hit
CAGCTCACTGTTTACG55890.18809233183000815No Hit
TGTTCCTCTTTATGGC54820.18449135142102427No Hit
AGCCTACCTTAACTCG54640.18388557901577465No Hit
CAATGTGACCAACGCC50840.1710970504605048No Hit
CTGCCTTGACCATAAC49670.16715952982638227No Hit
AGCTTGTGACTCACTC49270.16581336892582754No Hit
CCTGTTGACCCTAGTA49060.1651066344530363No Hit
CACAGCTCTGGACTGA48960.16477009422789765No Hit
TAACCAGACATCACAC48560.16342393332734292No Hit
CATGCAAACGAAGGCT47650.16036141727858091No Hit
CATGATGTGTCATGTG47510.1598902609633868No Hit
ATGGGTACTTTCGGGC46640.15696236100468025No Hit
CTATTCAACCGAACGA46510.15652485871199998No Hit
ATGCCGCGATGCCACG46370.15605370239680583No Hit
TTGTTTGTGATACGCT43980.14801039101599137No Hit
ATTAGCGTGGTCGGTC43690.14703442436308922No Hit
CATTAGTCTATTCTGG43650.14689980827303373No Hit
AGAACGCGAGGAATAT43580.14666423011543667No Hit
CACTATACTACGCCAC43170.14528441519236807No Hit
GCATCTCTGAGCCTAT42010.1413805485807594No Hit
GCAGATACTCGGCTCA41270.13889015091473317No Hit
CCGCTATTGTTCGCAA41080.13825072448696965No Hit
GGGTCGTACCGCTCGA40570.1365343693387624No Hit
ATAGGAAACAGACTTC39740.13374108547011135No Hit
AGCTCGGACCTTAAGC39640.1334045452449727No Hit
AACGTCCCTTACAGAA39350.13242857859207052No Hit
TGGGCCATGCGTTCTC39300.13226030847950115No Hit
CCCAATGCTGGAATGC38940.13104876366900192No Hit
GGGATCAGATGAGTAA38750.13040933724123843No Hit
TTGTAACCTCATCCTC38730.13034202919621068No Hit
TGGAGTTACAAGTCTC38540.1297026027684472No Hit
CTGCCATTGCACAGTT38270.12879394416057274No Hit
CTGATTACTGCTTTGA38250.12872663611554502No Hit
AACCAACACCGGAATG38210.12859202002548956No Hit
ATAGCCAACATGGCCC38170.1284574039354341No Hit
TCAGGCTCTTAAAGCA37950.127717015440129No Hit
TTGACGTGACCTCAAA37820.1272795131474487No Hit
GATACCATGTCTGGAT37740.12701028096733777No Hit
CCTACGGGATGGAACG37650.12670739476471293No Hit
CGCTTACTGGCCACGT36700.1235102626258955No Hit
CGATCAAACCGATCGC36550.12300545228818748No Hit
CTCGGATCTTTCTGAG35800.12048140059964738No Hit
TGTTTGTTGCGCTCGA35560.11967370405931455No Hit
TCCAAAGTGAAGGCCA35420.1192025477441204No Hit
ATCTTACTGCGCACTG35270.11869773740641237No Hit
TTCTAACACTACGTTG34920.11751984661842699No Hit
CGCTTCCCTGCGCTGT34670.1166784960555803No Hit
GCGTCGATGCTTGAAC34510.1161400316953584No Hit
CATGTTACTAACGTGG34210.11513041101994237No Hit
CTTAGGCCTTATGACC34140.11489483286234528No Hit
GTAAGAAACGACAAAT34050.11459194665972047No Hit
TGGGTGCACTTCTGAG33950.1142554064345818No Hit
CGCTGAGTGTTAGCTG33820.11381790414190152No Hit
GGTAGCCTGTGCCACG33350.11223616508374973No Hit
TACAACCGATTCCATT33280.11200058692615264No Hit
TACGCCTTGGGTTGAG33190.11169770072352783No Hit
CGAGTCTCTTACTTTC33120.11146212256593076No Hit
AGTGGTACTCAACGCC33010.1110919283182782No Hit
ATTGAGAGATGATCAC32860.11058711798057018No Hit
ATCGCAATGTAACTCG32340.10883710880984906No Hit
TTCACGCTGTGGGCAA32200.1083659524946549No Hit
TATCTCCCTGGAATTA32140.10816402835957169No Hit
CCTCCCTTGTTTCGCC32070.10792845020197461No Hit
CCTTCAGTGTAGCTTG31990.10765921802186368No Hit
GCATATCGAGCCGCTA31540.10614478700873961No Hit
GCGACAAGAAAGGCTG31520.10607747896371188No Hit
GCATAGTACGCTCCTT31510.10604382494119802No Hit
CATGTCAGAACAGGTG31450.1058419008061148No Hit
TGAGATAGACTCATCC31390.10563997667103159No Hit
GTGTACTGAATCACAC31300.10533709046840678No Hit
AACAGCTACTCGAGAG31150.10483228013069876No Hit
AGAACAATGAGTCGGA31100.10466401001812942No Hit
AACCACTGAAAACATG30930.10409189163539366No Hit
GAAAGTAACCCTGCGA30870.10388996750031046No Hit
GGGCTGACTAATACAG30720.10338515716260244No Hit
AGCCGGTACGCGCAAA30680.10325054107254697No Hit
GGTCAGAGAACCAGTT30650.10314957900500538No Hit
TGGTCCTTGTTTCTGT30330.10207265028456158No Hit
AGGTTACTGGTCCCGA30260.10183707212696451No Hit
ACCTCCATGGAATTGA30050.10113033765417327No Hit
TTCTATGACCAACGCC29800.10028898709132658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)