FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3814753
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGCA373810897.99082666689036No Hit
GTGGCGCA137950.3616223645410332No Hit
GGGGAGCA67750.17759996518778542No Hit
GAGGAGCA64790.16984061615522683No Hit
GTGGAGAA56770.14881697451971332No Hit
GTGGAGCT54980.14412466547637554No Hit
GTGCAGCA47240.12383501631691488No Hit
GTGGAGCC44010.11536788882530534No Hit
GTGGACCA40460.10606191278963539ABI Solid3 EF1 alpha Antisense Primer (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)