FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008440
Sequences flagged as poor quality0
Sequence length8
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAGGA291120196.76779327491988No Hit
GGCAAGGC164290.5460969804948744No Hit
GGCAAGAA95650.31793886532555077No Hit
GGCAACGA89070.29606706465809524No Hit
GGCAAGCA75020.2493651194639082Clontech Universal Primer Mix Long (100% over 8bp)
GCCAAGGA61550.20459108375104704RNA PCR Primer, Index 1 (100% over 8bp)
CGCAAGGA58740.19525069471220965No Hit
GGAAAGGA50400.16752868596348938No Hit
GGCAGGGA43390.14422757309436118No Hit
GGGAAGGA35750.1188323516506894No Hit
GGCAATGA31280.10397415271702276No Hit
GGCAAGTA31200.10370823416787438No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)