FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19167244
Sequences flagged as poor quality0
Sequence length34
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG218130.11380352856153966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG30000.08.8197565
TAGACTG24850.08.619485
TATACTG22150.08.53249555
TACACCG9100.08.4613045
TAGACAG29850.08.44197755
TAGACCG6500.08.3997675
GTGTAGG27600.08.2722041
CCTACAC29050.08.240373
CTAGTAC12950.08.1075243
CTAGACT24650.08.0642224
GTCCTAG26000.08.0270441
TCTACAC25450.07.975853
CTAGGAC29350.07.9653553
CCTATAC16200.07.9500443
TCTAGAC21200.07.92395733
TAGTACT17150.07.91769364
GTCTTAG21850.07.88490441
CCAGGAC59050.07.65736533
CTACACT31050.07.5742354
TAGGACT25350.07.56543164
Produced by FastQC (version 0.11.5)