FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5244344
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGTACT510091797.26511075551107No Hit
ATCGTACA240120.45786470147648595No Hit
ATAGTACT165130.3148725560337003No Hit
ATCGAACT133080.2537590974200014No Hit
AACGTACT118880.22668230764419725No Hit
ATCGTAAT84720.16154546688775565No Hit
ATCCTACT84580.1612785126223604No Hit
CTCGTACT73650.14043701175971676No Hit
ATCGTAGT61060.11643019603595799No Hit
ATCGTCCT59700.11383692602926124No Hit
ATCGTACG58210.11099576991898319RNA PCR Primer, Index 22 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)