FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004946913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3696926
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTCCT359748697.31019771561562No Hit
TCACTCCA228110.6170261455057526No Hit
TCCCTCCT123930.3352244540464159No Hit
ACACTCCT114040.30847249850281017No Hit
TCACACCT71430.19321457881493975No Hit
TCACTACT70040.18945469830881115No Hit
TAACTCCT52520.14206397423156428No Hit
TCAATCCT41670.11271526668372588No Hit
TCACTCAT40070.10838734667667138RNA PCR Primer, Index 45 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)