FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004946914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596850
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT660451.8361899995829685No Hit
GAAGCAGTGGAACCTT218540.6075871943506123No Hit
GTTCTTCCTAACGTAA183520.5102242239737548No Hit
CATGGAGGATCGACAT151070.42000639448406246No Hit
CGCTTAAGATAATAGG150920.4195893629147726No Hit
TGTCCCATGAGACAAT146930.4084963231716641No Hit
GATACGTACCGCTCGA143210.3981539402532772No Hit
AAAGCATGATCTTTGT138270.38441970057133323No Hit
TGAGCGCACAGGACAC130230.36206680845740025No Hit
TGTCGAAACTTCGCAA125200.3480823498338824No Hit
TAATTGCCTGTCTGGC120280.33440371436117716No Hit
GGGGGGGGGGGGGGGG119710.33281899439787593No Hit
GGAAGGACTAGGTAAT103470.2876683764961007No Hit
AATGATGGAAAGCTAA98650.2742677620695887No Hit
CATGTCAGAGGTTTGT98330.27337809472177044No Hit
TCGACCTTGTTTCGCC91310.253861017279008No Hit
ATGGGTACTTGGCTGC75540.21001709829434087No Hit
GTGAGATCTCGATTAC75530.20998929618972154No Hit
AAACGACCTAGGGAGG70240.19528198284610146No Hit
TTGCGATGACATTCAG69810.19408649234747072No Hit
AGCCTACCTTAACTCG68070.18924892614370908No Hit
TGTTCCTCTTTATGGC66870.18591267358939073No Hit
GCTATGATGACCTGCG66610.1851898188692884No Hit
CAGCTCACTGTTTACG66370.18452256835842473No Hit
CCTGTTGACCCTAGTA64390.17901775164379943No Hit
CTGCCTTGACCATAAC64290.17873973059760626No Hit
CAATGTGACCAACGCC62000.17237304863978203No Hit
CATGCAAACGAAGGCT60680.16870317083003183No Hit
TAACCAGACATCACAC60140.16720185718058858No Hit
CATGATGTGTCATGTG60110.1671184508667306No Hit
CACAGCTCTGGACTGA59170.16450505303251456No Hit
ATGCCGCGATGCCACG59060.16419922988170205No Hit
ATGGGTACTTTCGGGC57930.16105759205971892No Hit
CTATTCAACCGAACGA57490.15983429945646888No Hit
TTGTTTGTGATACGCT55320.15380124275407647No Hit
AGAACGCGAGGAATAT55200.15346761749864465No Hit
TGGGCCATGCGTTCTC54970.1528281690924003No Hit
CATTAGTCTATTCTGG54420.1512990533383377No Hit
ATTAGCGTGGTCGGTC53870.14976993758427515No Hit
AGCTTGTGACTCACTC52640.14635027871609882No Hit
CCCAATGCTGGAATGC50740.14106787883842806No Hit
CCGCTATTGTTCGCAA50470.14031722201370644No Hit
CACTATACTACGCCAC50330.13992799254903598No Hit
GGGCTGACTAATACAG49830.13853788731806999No Hit
AACCAACACCGGAATG48790.13564646843766073No Hit
ATAGGAAACAGACTTC48440.13467339477598453No Hit
AACGTCCCTTACAGAA48390.13453438425288794No Hit
TTGACGTGACCTCAAA48190.13397834216050153No Hit
AGCTCGGACCTTAAGC47900.13317208112654128No Hit
TTGTAACCTCATCCTC47540.13217120536024576No Hit
GGTAGCCTGTGCCACG47350.13164296537247872No Hit
CTGCCATTGCACAGTT47050.1308089022338991No Hit
GCAGATACTCGGCTCA46970.13058648539694453No Hit
CCTACGGGATGGAACG46830.13019725593227408No Hit
GCATCTCTGAGCCTAT46420.12905736964288197No Hit
CGCTTACTGGCCACGT45760.12722243073800688No Hit
TGGAGTTACAAGTCTC45720.1271112223195296No Hit
CTGATTACTGCTTTGA45690.12702781600567162No Hit
ATAGCCAACATGGCCC45530.12658298233176252No Hit
CGCTGAGTGTTAGCTG45470.1264161697040466No Hit
GGGATCAGATGAGTAA45060.1252762834146545No Hit
TGTTTGTTGCGCTCGA44270.12307991714972824No Hit
TCAGGCTCTTAAAGCA44170.12280189610353505No Hit
CGATCAAACCGATCGC44140.12271848978967707No Hit
ATCTTACTGCGCACTG43830.12185662454647816No Hit
CGCTTCCCTGCGCTGT43580.12116157193099518No Hit
CATGTTACTAACGTGG43240.12021630037393832No Hit
TCCAAAGTGAAGGCCA43080.1197714667000292No Hit
TTCTAACACTACGTTG42220.1173804857027677No Hit
CTCGGATCTTTCTGAG42000.11676883940114266No Hit
GATACCATGTCTGGAT41590.11562895311175057No Hit
TGGGTGCACTTCTGAG41450.1152397236470801No Hit
AGCCGGTACGCGCAAA41170.11446126471773913No Hit
CCTTCAGTGTAGCTTG41000.11398862893921069No Hit
CCTCCCTTGTTTCGCC40590.1128487426498186No Hit
TACAACCGATTCCATT40420.11237610687129015No Hit
CGAGTCTCTTACTTTC40400.11232050266205151No Hit
CTTAGGCCTTATGACC40370.11223709634819357No Hit
AGTGGTACTCAACGCC40320.11209808582509696No Hit
ACCTGCTTGCCAAACT39770.11056897007103436No Hit
TACGCCTTGGGTTGAG39740.11048556375717641No Hit
AACAGCTACTCGAGAG39730.11045776165255711No Hit
ATTGAGAGATGATCAC39510.10984611535093207No Hit
TATCTCCCTGGAATTA39480.10976270903707412No Hit
CATGTCAGAACAGGTG39160.10887304168925586No Hit
ATCGCAATGTAACTCG39160.10887304168925586No Hit
AGAACAATGAGTCGGA38690.10756634277214785No Hit
GTCCTGACTTTACGAC38530.10712150909823874No Hit
TGAGATAGACTCATCC38380.10670447752894893No Hit
GGGTCGTACCGCTCGA38140.10603722701808527No Hit
TTCACGCTGTGGGCAA38100.10592601859960799No Hit
AGAACATGAAAGCTAA38070.10584261228575004No Hit
GTGTACTGAATCACAC38040.10575920597189208No Hit
TGGTCCTTGTTTCTGT38030.10573140386727276No Hit
AACCACTGAAAACATG37980.10559239334417614No Hit
GCATGGGCTATGTGGG37840.10520316387950567No Hit
ACCTCCATGGAATTGA37680.10475833020559656No Hit
TCCGGGATGCGAGTTT37410.10400767338087492No Hit
TTCTATGACCAACGCC37300.10370185023006241No Hit
GTTGCTAGACGCTCGA37140.1032570165561533No Hit
AGGTTACTGGTCCCGA36810.10233954710371575No Hit
CCTTCTTTGGATCATC36760.10220053658061916No Hit
CTAGAGACTCCGTTAA36360.10108845239584638No Hit
ATTCCTAACATTGCCA36340.10103284818660772No Hit
CGAATTTCTGATAGCG36300.10092163976813044No Hit
TTCGACCACAGTACGG36190.10061581661731792No Hit
GTAAGAAACGACAAAT36160.10053241030345997No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)