FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004946918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3781513
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTCCTT362417295.83920510123858No Hit
CTTTCCTA684961.8113384774824257No Hit
CTTTCCAT105200.27819552649957835No Hit
CTTTACTT87710.23194419799693933No Hit
CTTTCATT84580.22366708775032637No Hit
CGTTCCTT79760.2109208668593761No Hit
CATTCCTT74700.1975399793680466TruSeq Adapter, Index 27 (100% over 8bp)
CTATCCTT69740.18442353629354177No Hit
ATTTCCTT63320.16744620473339641No Hit
CTTACCTT50160.13264531947926664No Hit
CTTTCCGT45480.12026932077187093No Hit
CTGTCCTT41750.11040554402430985No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)