FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004946921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3130715
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGCCGACCAGGGAT574971.8365453259079796No Hit
GAAGCAGTGGAACCTT191120.6104675768953737No Hit
GTTCTTCCTAACGTAA162760.5198812411861188No Hit
CGCTTAAGATAATAGG133460.4262923964653441No Hit
CATGGAGGATCGACAT129600.41396294456697597No Hit
TGTCCCATGAGACAAT126100.40278338973684924No Hit
GATACGTACCGCTCGA122400.3909650032021439No Hit
AAAGCATGATCTTTGT119020.3801687473947644No Hit
TGAGCGCACAGGACAC114850.3668491063542993No Hit
TGTCGAAACTTCGCAA108060.34516076998385353No Hit
GGGGGGGGGGGGGGGG107250.3425735015803099No Hit
TAATTGCCTGTCTGGC104490.33375762405712434No Hit
GGAAGGACTAGGTAAT91610.2926168622822582No Hit
AATGATGGAAAGCTAA86330.27575170528138143No Hit
CATGTCAGAGGTTTGT84680.27048134371860744No Hit
TCGACCTTGTTTCGCC79280.2532328876949834No Hit
ATGGGTACTTGGCTGC68320.21822491028407248No Hit
GTGAGATCTCGATTAC67230.21474327749411876No Hit
AAACGACCTAGGGAGG63070.2014555780388825No Hit
AGCCTACCTTAACTCG60970.19474784514080648No Hit
TTGCGATGACATTCAG59910.19136203710653957No Hit
GCTATGATGACCTGCG57880.1848778953050661No Hit
TGTTCCTCTTTATGGC57670.1842071220152585No Hit
CAGCTCACTGTTTACG56440.18027830703209968No Hit
CTGCCTTGACCATAAC54830.17513571181024143No Hit
CCTGTTGACCCTAGTA54610.1744329969352049No Hit
CAATGTGACCAACGCC54320.17350669096356583No Hit
CACAGCTCTGGACTGA53270.17015282451452785No Hit
CATGCAAACGAAGGCT52980.16922651854288875No Hit
TAACCAGACATCACAC52540.1678210887928157No Hit
ATGCCGCGATGCCACG51750.16529770355972997No Hit
CATGATGTGTCATGTG51370.1640839233210305No Hit
ATGGGTACTTTCGGGC51100.1632215005198493No Hit
TGGGCCATGCGTTCTC50740.1620716034516077No Hit
AGAACGCGAGGAATAT48440.15472503884895303No Hit
TTGTTTGTGATACGCT48200.15395844080345863No Hit
CTATTCAACCGAACGA47560.1519141793488069No Hit
CCGCTATTGTTCGCAA46360.14808118912133492No Hit
ATTAGCGTGGTCGGTC46080.14718682473492478No Hit
CATTAGTCTATTCTGG45880.1465479930303461No Hit
AGCTTGTGACTCACTC45870.14651605144511717No Hit
CACTATACTACGCCAC44250.14134151463802996No Hit
GGGCTGACTAATACAG43090.1376362907514737No Hit
CCCAATGCTGGAATGC43000.1373488164844133No Hit
TTGACGTGACCTCAAA42350.13527261344453265No Hit
ATAGGAAACAGACTTC42330.13520873027407476No Hit
AACCAACACCGGAATG42220.1348573728365565No Hit
GGTAGCCTGTGCCACG42170.13469766491041185No Hit
AACGTCCCTTACAGAA41980.13409077479106213No Hit
AGCTCGGACCTTAAGC41380.1321742796773261No Hit
CCTACGGGATGGAACG41270.13182292223980785No Hit
CTGCCATTGCACAGTT40860.1305133172454216No Hit
ATAGCCAACATGGCCC40790.13028972614881904No Hit
GCAGATACTCGGCTCA40710.13003419346698758No Hit
GCATCTCTGAGCCTAT40050.12792604884187797No Hit
CTGATTACTGCTTTGA39820.1271913923816125No Hit
TGGAGTTACAAGTCTC39780.1270636260406968No Hit
TTGTAACCTCATCCTC39730.12690391811455212No Hit
CGCTTACTGGCCACGT39570.12639285275088918No Hit
CGATCAAACCGATCGC39200.12521101409741864No Hit
TCCAAAGTGAAGGCCA38680.12355005166551411No Hit
TGTTTGTTGCGCTCGA38480.12291121996093543No Hit
CGCTGAGTGTTAGCTG38470.12287927837570652No Hit
TCAGGCTCTTAAAGCA38380.12259180410864612No Hit
GGGATCAGATGAGTAA38080.12163355655177811No Hit
ATCTTACTGCGCACTG37970.12128219911425983No Hit
GATACCATGTCTGGAT37940.12118637435857305No Hit
CATGTTACTAACGTGG37420.1195254119266685No Hit
CGCTTCCCTGCGCTGT37320.11920599607437916No Hit
TTCTAACACTACGTTG37170.11872687229594518No Hit
CTCGGATCTTTCTGAG36710.11725755937541424No Hit
TGGGTGCACTTCTGAG36640.11703396827881171No Hit
CTTAGGCCTTATGACC35930.11476611572755745No Hit
CCTTCAGTGTAGCTTG35900.11467029097187065No Hit
AGTGGTACTCAACGCC35810.11438281670481025No Hit
AGCCGGTACGCGCAAA35530.11348845231840012No Hit
CGAGTCTCTTACTTTC35350.1129135037842793No Hit
CCTCCCTTGTTTCGCC35260.1126260295172189No Hit
TACAACCGATTCCATT34830.11125254135237478No Hit
TACGCCTTGGGTTGAG34300.10955963733524131No Hit
AACAGCTACTCGAGAG34050.10876109770451797No Hit
TATCTCCCTGGAATTA33900.10828197392608399No Hit
ACCTGCTTGCCAAACT33870.10818614917039718No Hit
AGAACAATGAGTCGGA33840.10809032441471038No Hit
ATTGAGAGATGATCAC33810.10799449965902357No Hit
TGGTCCTTGTTTCTGT33710.10767508380673425No Hit
TCCGGGATGCGAGTTT33650.10748343429536064No Hit
ATCGCAATGTAACTCG33130.10582247186345611No Hit
AACCACTGAAAACATG33060.10559888076685357No Hit
TGAGATAGACTCATCC33030.10550305601116677No Hit
TTCACGCTGTGGGCAA32860.10496004906227492No Hit
GTCCTGACTTTACGAC32860.10496004906227492No Hit
ACCTCCATGGAATTGA32840.10489616589181704No Hit
GCATGGGCTATGTGGG32820.1048322827213592No Hit
AAAGGATACTGCTGGC32740.10457675003952771No Hit
CATGTCAGAACAGGTG32710.10448092528384091No Hit
GGGTCGTACCGCTCGA32390.10345879455651505No Hit
GTGTACTGAATCACAC32370.10339491138605718No Hit
CCTTCTTTGGATCATC32180.10278802126670744No Hit
AGAGAATTGCCCGCAA31920.10195754005075518No Hit
TTCTATGACCAACGCC31850.10173394895415264No Hit
GTAAGAAACGACAAAT31800.10157424102800797No Hit
ATTCCTAACATTGCCA31790.10154229944277905No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)