FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004946928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004946928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17153382
Sequences flagged as poor quality0
Sequence length34
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC16250.011.11465322
TATACTG21100.09.1554885
ATCTCCG20800.09.15274910
CACGAGA19700.09.09631221
TCTCCGA21450.09.00591411
TATACAC36200.08.8943243
CCCACGA20550.08.78790919
TACACCG8150.08.7598665
CTTATAC24700.08.5603251
TAGACAG29800.08.502515
TAGACTG25900.08.3775455
ACACCGT9750.08.18382556
GTATAGA17900.08.1356171
TACACTG33250.08.04130655
CCTACAC24500.07.99936773
GACTCTA24250.07.91155926
CTAGTAC12650.07.74642323
TCTATAC15200.07.7362313
TAATACT18950.07.6825914
GTATTAG17950.07.6449011
Produced by FastQC (version 0.11.5)